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AB233752

Anti-BTN2A2/BTF2抗体[4B11C8]

Anti-BTN2A2/BTF2 antibody [4B11C8]

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(2 Publications)

Mouse Monoclonal BTN2A2/BTF2 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BTN2A2 aa 50-250.

查看别名

BT2.2, BTF2, BTN2A2, Butyrophilin subfamily 2 member A2

5 Images
Flow Cytometry - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BTN2A2/BTF2 with ab233752 at 1/200 dilution (green) compared with a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

HeLa (human prostate adenocarcinoma cell line) cells stained for BTN2A2/BTF2 (green) using ab233752 at 1/200 dilution in ICC/IF.

Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • WB

Supplier Data

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

All lanes:

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution

All lanes:

K562 cell lysate

Predicted band size: 59 kDa

false

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • WB

Supplier Data

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

Lanes 1 - 2:

Western blot - Anti-BTN2A2/BTF2 antibody [6C7D2] (<a href='/products/primary-antibodies/btn2a2-btf2-antibody-6c7d2-ab233763'>ab233763</a>) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-GCSF Receptor antibody [8F8B12] (<a href='/products/primary-antibodies/gcsf-receptor-antibody-8f8b12-ab233783'>ab233783</a>) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

GCSF Receptor (AA: extra 25-187)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 59 kDa,92 kDa

true

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • WB

Supplier Data

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

All lanes:

Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution

All lanes:

Human BTN2A2/BTF2 (aa 57-237) recombinant protein

Predicted band size: 59 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4B11C8

亚型

IgG2a

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF, Flow Cyt

applications

免疫原

Recombinant Fragment Protein within Human BTN2A2 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q8WVV5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
Purified from tissue culture supernatant.
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The BTN2A2/BTF2 protein also known as Butyrophilin Subfamily 2 Member A2 plays an important role in immune regulation. It has a molecular mass of approximately 57 kDa. BTN2A2 is part of the butyrophilin family and is expressed mainly in immune-related cells including those found in lymphoid tissues. The protein is a transmembrane glycoprotein with a conserved B30.2 domain which is a characteristic feature of the butyrophilin family.
Biological function summary

BTN2A2/BTF2 modulates T cell activity helping to maintain immune tolerance and preventing overactive immune responses. It functions as part of a larger complex that includes various other immunoregulatory proteins. This complex is critical for fine-tuning immune responses by affecting T cell proliferation and cytokine production. Without proper regulation by BTN2A2 there can be an increased risk of autoimmunity and immune dysregulation.

Pathways

BTN2A2/BTF2 involvement is essential within the immune checkpoint pathways particularly those influencing T cell regulation. It interacts closely with the PD-1 pathway which is significant in mediating immune inhibitory signals. Furthermore BTN2A2 has relationships with other proteins in these pathways such as CTLA-4 which is another key player in maintaining immune balance.

BTN2A2/BTF2 is implicated in autoimmune conditions like rheumatoid arthritis where the immune system attacks healthy tissue. The dysregulation of BTN2A2 might exacerbate these conditions. It also shows associations with inflammatory bowel disease (IBD) where improper immune regulation plays a central role. BTN2A2’s interaction with proteins like PD-1 in these diseases suggests potential therapeutic targets for modulating immune responses and improving patient outcomes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.
See full target information BTN2A2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:816760 PubMed36033440

2022

Comprehensive Analysis of the Prognostic Value and Immune Infiltration of Butyrophilin Subfamily 2/3 (BTN2/3) Members in Pan-Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Dong He,Zhen Qin,Zihao Liu,Xiaoshuai Ji,Jiajia Gao,Hua Guo,Fan Yang,Haitao Fan,Yanbang Wei,Zixiao Wang,Qian Liu,Qi Pang

Clinical cancer research : an official journal of the American Association for Cancer Research 28:2020-2029 PubMed35165101

2022

Praluzatamab Ravtansine, a CD166-Targeting Antibody-Drug Conjugate, in Patients with Advanced Solid Tumors: An Open-Label Phase I/II Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Boni,Mary J Fidler,Hendrik-Tobias Arkenau,Alexander Spira,Funda Meric-Bernstam,Nataliya Uboha,Rachel E Sanborn,Randy F Sweis,Patricia LoRusso,Misako Nagasaka,Javier Garcia-Corbacho,Shadia Jalal,James J Harding,Stella K Kim,Iris H C Miedema,Danielle J Vugts,Marc C Huisman,Gerben J C Zwezerijnen,Guus A M S van Dongen,C Willemien Menke van der Houven van Oordt,Song Wang,Tam Dang,Ivan A Zein,Olga Vasiljeva,Susan K Lyman,Virginia Paton,Alison Hannah,Joyce F Liu
View all publications
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