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AB326054

Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector)

Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CD272/BTLA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA

3 Images
Sandwich ELISA - Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector) (AB326054)
  • sELISA

Lab

Sandwich ELISA - Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector) (AB326054)

Interpolated concentration of native CD272/BTLA was measured at different sample concentrations and the interpolated dilution factor corrected values (to neat sample). Undiluted sample is as follows : Human serum and Human plasma (EDTA) 100%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector) (AB326054)
  • sELISA

Lab

Sandwich ELISA - Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector) (AB326054)

Sandwich ELISA using Human CD272/BTLA immunogen protein standard curve with the capture antibody ab326053 dilution at 2 µg/mL and detector antibody ab326054 dilution at 0.5 µg/mL. The background-subtracted absorbance data values are presented as mean +/- standard deviation

Sandwich ELISA - Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector) (AB326054)
  • sELISA

Lab

Sandwich ELISA - Anti-BTLA antibody [EPR29878-40] - BSA and Azide free (Detector) (AB326054)

Interpolated concentration of native CD272/BTLA was measured at different sample concentrations. Undiluted sample is as follows : Human Tonsil extract (0.25mg/ml) . Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29878-40

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

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Primary Antibodies

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Primary Antibodies

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Anti-TIM 3 antibody [EPR22241]

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  • 4
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab326054 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human BTLA. The recommended pair for sandwich ELISA is:

Capture: ab326053, Human BTLA Capture Antibody (unconjugated)

Detector: ab326054, Human BTLA Detector Antibody (unconjugated)

The reference range value is 3.9-1000pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 12796776, PubMed : 14652006, PubMed : 15568026, PubMed : 18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed : 19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed : 19915044).
See full target information BTLA
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Abcam Product Promise

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