重组Anti-BTK (phospho Y551)抗体[EP267Y] (ab40770)
![Indirect ELISA - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.cn/ps/products/40/ab40770/Images/ab40770-487856-anti-btk-phospho-Y551-antibody-ep267Y-elisa-human.png "Indirect ELISA - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP267Y] to BTK (phospho Y551)
- Suitable for: ICC/IF, Indirect ELISA, WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-BTK (phospho Y551)抗体[EP267Y]
参阅全部 BTK 一抗 -
描述
兔单克隆抗体[EP267Y] to BTK (phospho Y551) -
宿主
Rabbit -
特异性
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
ab40770 also reacts with family proteins including BMX (phospho Y566), ITK (phospho Y512), TEC (phospho Y519) and TXK (phospho Y420).
-
经测试应用
适用于: ICC/IF, Indirect ELISA, WB, Dot blotmore details
不适用于: Flow Cyt,IHC or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Ramos treated with 1mM pervanadate whole cell lysates, K-562 treated with 1mM pervanadate whole cell lysate ICC/IF: Ramos cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP267Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40770于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500.
|
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
|
| WB |
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
|
| Dot blot |
1/1000.
|
| 说明 |
|---|
|
ICC/IF
1/500. |
|
Indirect ELISA
Use at an assay dependent concentration. |
|
WB
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
|
Dot blot
1/1000. |
靶标
-
功能
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
疾病相关
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
细胞定位
Cytoplasm. Membrane. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 695 Human
- Omim: 300300 Human
- SwissProt: Q06187 Human
- Unigene: 159494 Human
-
别名
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
图片
-
Indirect ELISA - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
ELISA using ab40770 at varying antibody concentrations and antigen concentration of 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody. Substrate solution was p-nitrophenyl phosphate (PNPP).
-
![Dot Blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.cn/ps/products/40/ab40770/Images/ab40770-487835-anti-btk-phospho-Y551-antibody-ep267Y-dot-blot-human.jpg)
Dot Blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)Dot blot analysis of Human BTK non-phospho peptide (lane 1), Human BTK Y551 phospho peptide (lane 2), Human BMX non-phospho peptide (lane 3), Human BMX Y566 phospho peptide (lane 4), Human ITK non-phospho peptide (lane 5), Human ITK Y512 phospho peptide (lane 6), Human TEC non-phospho peptide (lane 7), Human TEC Y519 phospho peptide (lane 8), Human TXK non-phospho peptide (lane 9) and Human TXK Y420 phospho peptide (lane 10) with ab40770 at a dilution of 1/1000. ab97051 Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/20,000.
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
Exposure time: 180 seconds
-
![Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.cn/ps/products/40/ab40770/Images/ab40770-487833-anti-btk-phospho-Y551-antibody-ep267Y-western-blot-2-human.jpg)
Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/1000 dilution
Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : K-562 treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : K-562 treated with 1mM pervanadate for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
-
![Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.cn/ps/products/40/ab40770/Images/ab40770-487831-anti-btk-phospho-Y551-antibody-ep267Y-western-blot-1-human.jpg)
Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/1000 dilution
Lane 1 : Untreated Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
-
![Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.cn/ps/products/40/ab40770/Images/ab40770-487854-anti-btk-phospho-Y551-antibody-ep267Y-immunocytochemistry-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma B lymphocyte) cells labelling BTK with primary antibody anti- BTK (phospho Y551) (ab40770) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) secondary antibody at 1/1000 dilution (2.0 µg/mL). Confocal image showing increased cytoplasmic staining in Ramos cells treated with pervanadate (1 mM) for 30 min and then the signal decreased after Alkaline Phosphatase treatment 37℃ for 1 hour. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL). The nuclear counter stain is DAPI (blue).
The secondary antibody only control is : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2.0 µg/mL).
-
![Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.cn/ps/products/40/ab40770/Images/ab40770-4-benefits-of-recombinant-antibodies.png)
Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab40770 被引用在 6 文献中.
- Shu ST et al. Visualization of Host Cell Kinase Activation by Viral Proteins Using GFP Fluorescence Complementation and Immunofluorescence Microscopy. Bio Protoc 11:e4068 (2021). PubMed: 34327265
- Li WF et al. HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane. J Biol Chem 295:5163-5174 (2020). PubMed: 32144207
- Mo ZQ et al. Molecular characterization and function analysis of grouper (Epinephelus coioides) Bruton's tyrosine kinase BTK. Fish Shellfish Immunol 77:91-99 (2018). WB . PubMed: 29567142
- Rushworth SA et al. Identification of Bruton's tyrosine kinase as a therapeutic target in acute myeloid leukemia. Blood 123:1229-38 (2014). Human . PubMed: 24307721
- Daniel JL et al. Cbl-b is a novel physiologic regulator of glycoprotein VI-dependent platelet activation. J Biol Chem 285:17282-91 (2010). PubMed: 20400514
- Grasis JA et al. In vivo significance of ITK-SLP-76 interaction in cytokine production. Mol Cell Biol 30:3596-609 (2010). PubMed: 20457812