重组Anti-BTK抗体[Y440] (ab32555)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y440] to BTK
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BTK抗体[Y440]
参阅全部 BTK 一抗 -
描述
兔单克隆抗体[Y440] to BTK -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human BTK aa 600-700 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: Daudi cell lysate, K-562 cell lysate, U-937 cell lysate.
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常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y440 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32555于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000. Predicted molecular weight: 76 kDa.
|
IP |
1/30.
|
说明 |
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WB
1/1000. Predicted molecular weight: 76 kDa. |
IP
1/30. |
靶标
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功能
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
疾病相关
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
细胞定位
Cytoplasm. Membrane. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 695 Human
- Omim: 300300 Human
- SwissProt: Q06187 Human
- Unigene: 159494 Human
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别名
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
图片
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All lanes : Anti-BTK antibody [Y440] (ab32555) at 1/1000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
BTK was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10 µg with ab32555 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10 µg
Lane 2: ab32555 IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32555 in Daudi whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab32555 被引用在 1 文献中.
- Alflen A et al. Idelalisib impairs TREM-1 mediated neutrophil inflammatory responses. Sci Rep 8:5558 (2018). PubMed: 29615799