Anti-BTAF1抗体[BTA3D61] (ab50990)
![Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)](https://www.abcam.cn/ps/products/50/ab50990/Images/ab50990-62806-anti-btaf1-antibody-bta3d61-western-blot.jpg "Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)")
Key features and details
- Mouse monoclonal [BTA3D61] to BTAF1
- Suitable for: WB, IP, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-BTAF1抗体[BTA3D61]
参阅全部 BTAF1 一抗 -
描述
小鼠单克隆抗体[BTA3D61] to BTAF1 -
宿主
Mouse -
经测试应用
适用于: WB, IP, ICC, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to BTAF1.
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阳性对照
- WB: HEK293, NIH3T3 and F2408 whole cell lysates. ICC/IF and FACS: HeLa cells. IP: RIPA lysate of HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
BTA3D61 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50990于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
|
|
| IP |
Use at an assay dependent concentration.
Use at 5µg/0.5mg of lysate. |
|
| ICC |
1/10.
|
|
| Flow Cyt |
1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/50. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa). |
|
IP
Use at an assay dependent concentration. Use at 5µg/0.5mg of lysate. |
|
ICC
1/10. |
|
Flow Cyt
1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 8 HEAT repeats.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 9044 Human
- Entrez Gene: 107182 Mouse
- Entrez Gene: 368042 Rat
- Omim: 605191 Human
- SwissProt: O14981 Human
- Unigene: 500526 Human
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别名
- ATP dependent helicase BTAF1 antibody
- ATP-dependent helicase BTAF1 antibody
- B-TFIID transcription factor associated 170 kDa subunit antibody
see all
图片
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Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 207 kDa -
![Immunocytochemistry - Anti-BTAF1 antibody [BTA3D61] (ab50990)](https://www.abcam.cn/ps/products/50/ab50990/Images/ab50990-62805-anti-btaf1-antibody-bta3d61-immunocytochemistry.jpg)
Immunocytochemistry - Anti-BTAF1 antibody [BTA3D61] (ab50990)HeLa cells stained with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200. -
![Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (ab50990)](https://www.abcam.cn/ps/products/50/ab50990/Images/ab50990-62804-anti-btaf1-antibody-bta3d61-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (ab50990)Ab50990 used at 5µg/0.5mg RIPA lysate of HeLa cells. The immunoprecipitate was subjected to Western blotting using ab50990 at 1/100. The ECL technique was used, and the secondary antibody was Mouse IgG at 1/2500. -
![Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (ab50990)](https://www.abcam.cn/ps/products/50/ab50990/Images/ab50990-62803-anti-btaf1-antibody-bta3d61-flow-cytometry.jpg)
Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (ab50990)Cells labelled with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/400 dilution. -
![Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)](https://www.abcam.cn/ps/products/50/ab50990/Images/ab50990-62802-anti-btaf1-antibody-bta3d61-western-blot.jpg)
Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 207 kDa -
![Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)](https://www.abcam.cn/ps/products/50/ab50990/Images/ab50990-62801-anti-btaf1-antibody-bta3d61-western-blot.jpg)
Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990)Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + F2408 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 207 kDa
实验方案
数据表及文件
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Datasheet download
文献 (0)
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