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重组RabMAb

重组Anti-BST2/Tetherin抗体[EPR23597-202] - BSA and Azide free (ab272175)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
  • Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23597-202] to BST2/Tetherin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Unconjugated

概述

  • 产品名称

    Anti-BST2/Tetherin抗体[EPR23597-202] - BSA and Azide free
    参阅全部 BST2/Tetherin 一抗

  • 描述

    兔单克隆抗体[EPR23597-202] to BST2/Tetherin - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, ICC/IF, IP, Flow Cytmore details
    不适用于: WB

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse spleen tissue. ICC/IF: EL.4 and mouse splenocyte cells. Flow Cyt: Mouse splenocytesl. IP: Mouse spleen tissue lysate; EL4 whole cell lysate.

  • 常规说明

    ab272175 is the carrier-free version of ab246508.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272175于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for WB.

靶标

  • 功能

    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.

  • 组织特异性

    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).

  • 序列相似性

    Belongs to the tetherin family.

  • 结构域

    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.

  • 翻译后修饰

    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.

  • 细胞定位

    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Bone marrow stromal antigen 2 antibody
      • Bone marrow stromal cell antigen 2 antibody
      • Bone marrow stromal cell antigen antibody
      • BST 2 antibody
      • BST-2 antibody
      • BST2 antibody
      • BST2_HUMAN antibody
      • CD 317 antibody
      • CD317 antibody
      • CD317 antigen antibody
      • HM1.24 antigen antibody
      • NPC A 7 antibody
      • Tetherin antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling BST2/Tetherin with ab246508 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen (PMID: 19903902).The section was incubated with ab246508 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Mouse splenocyte cells labelling BST2/Tetherin with ab246508 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in mouse splenocyte ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      Flow cytometric analysis of  Mouse splenocyte cells labelling BST2/Tetherin with ab246508 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab246508 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      BST2/Tetherin was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte), whole cell lysate with ab246508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246508 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug

      Lane 2: ab246508 IP in EL4 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246508 in EL4 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

      BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      Immunofluorescent analysis of 100% methanol-fixed EL.4 cells labelling BST2/Tetherin with ab246508 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in EL.4 cell line. 100% methanol fixation is recommended. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      BST2/Tetherin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab246508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246508 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse spleen tissue lysate 10ug

      Lane 2: ab246508 IP in Mouse spleentissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246508 in mouse spleen tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

      BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

      Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling BST2/Tetherin with ab246508 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse skeletal muscle (PMID: 19903902). The section was incubated with ab246508 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246508).

    • Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)
      Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free (ab272175)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab272175 尚未被引用在任何文献中。

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