Anti-BROX抗体(ab122174)
Key features and details
- Rabbit polyclonal to BROX
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BROX抗体 -
描述
兔多克隆抗体to BROX -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: LGNLVKNTLE KCQRENGFIY FQKIPTEAPQ LELKANYGLV EPIPFEFPPT SVQWTPETLA AFDLTKRPKD DSTKPKPEEE VKPVKEPDIK PQKDTGCYIS, corresponding to amino acids of Human BROX
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阳性对照
- ICC/IF: U-2 OS cells. IHC-P: Human urinary bladder tissue. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/250 - 1/500.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
| 说明 |
|---|
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/250 - 1/500. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
靶标
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序列相似性
Belongs to the BROX family.
Contains 1 BRO1 domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 148362 Human
- SwissProt: Q5VW32 Human
- Unigene: 552608 Human
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别名
- BRO1 domain and CAAX motif containing antibody
- BRO1 domain containing protein antibody
- BRO1 domain- and CAAX motif-containing protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-BROX antibody (ab122174)
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BROX antibody (ab122174)ab122174, at 1/500 dilution, staining BROX in paraffin-embedded human urinary bladder tissue by Immunohistochemistry.
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Western blot - Anti-BROX antibody (ab122174)All lanes : Anti-BROX antibody (ab122174) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab122174 尚未被引用在任何文献中。