重组Anti-Brevican抗体[EPR25743-59] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control : No staining on human kidney. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Brevican was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate 10 ug with ab285162 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab285162 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human hypothalamus tissue lysate 10 ug

Lane 2 : ab285162 IP in Human hypothalamus tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab285162 in Human hypothalamus tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 119 seconds

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (<a href='/products/primary-antibodies/brevican-antibody-epr25743-59-ab285162'>ab285162</a>)

Predicted band size: 99 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling Brevican with ab285162 at 1/50 (19.72 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Brevican with ab285162 at 1/2000 (0.295 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab285162 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling Brevican with ab285162 at 1/50 (19.72 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Negative control : No staining on rat kidney(PMID : 32559242) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling Brevican with ab285162 at 1/50 (19.72 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Negative control : No staining on mouse kidney (PMID : 32559242) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling Brevican with ab285162 at 1/100 (19.72 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/ml dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Brevican was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab285162 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab285162 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab285162 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab285162 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-Brevican antibody [EPR25743-59] (<a href='/products/primary-antibodies/brevican-antibody-epr25743-59-ab285162'>ab285162</a>)

Predicted band size: 99 kDa

false

• WB

Lab

Western blot - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 150-kDa full length and 55-kDa secreted isoform are observed. The MW observed is consistent with what has been described in the literature (PMID : 32559242, PMID : 10801887).

Bands below 55-kDa may due to degradation.

Negative control : kidney (PMID : 32559242).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 136 seconds

All lanes:

Western blot - Anti-Brevican antibody [EPR25743-59] (<a href='/products/primary-antibodies/brevican-antibody-epr25743-59-ab285162'>ab285162</a>) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 99 kDa

false

• WB

Lab

Western blot - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 150-kDa full length and 55-kDa secreted isoform are observed. The MW observed is consistent with what has been described in the literature (PMID : 32559242, PMID : 10801887).

Bands below 55-kDa may due to degradation.

Negative control : heart, kidney, spleen (PMID : 32559242).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Brevican antibody [EPR25743-59] (<a href='/products/primary-antibodies/brevican-antibody-epr25743-59-ab285162'>ab285162</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

false

• WB

Lab

Western blot - Anti-Brevican antibody [EPR25743-59] - BSA and Azide free (AB285169)

This data was developed using ab285162, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 150-kDa full length and 55-kDa secreted isoform are observed. The MW observed is consistent with what has been described in the literature (PMID : 32559242, PMID : 10801887).

Bands below 55-kDa may due to degradation.

Negative control : heart, spleen (PMID : 32559242).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Brevican antibody [EPR25743-59] (<a href='/products/primary-antibodies/brevican-antibody-epr25743-59-ab285162'>ab285162</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

false