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Anti-Brevican抗体(ab111719)

Reviews (1)Q&A (3)References (5)
Western blot - Anti-Brevican antibody (ab111719)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody (ab111719)

Key features and details

  • Rabbit polyclonal to Brevican
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Brevican抗体
    参阅全部 Brevican 一抗

  • 描述

    兔多克隆抗体to Brevican

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow

  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 83-262 of Human Brevican (AAH09117).

  • 阳性对照

    • Human breast carcinoma tissue; MCF7 whole cell lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab111719于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性

    Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C-terminal epitope EAMESE.

  • 细胞定位

    Isoform 1 is secreted, extracellular space, extracellular matrix; Isoform 2 is found on the membrane; Lipid-anchor, GPI-anchor.

  • 数据库链接

    see all

  • 别名

    • BCAN antibody
    • BEHAB antibody
    • Brain enriched hyaluronan binding protein antibody
    • Brevican core protein antibody
    • Brevican core protein isoform 1 antibody
    • Brevican core protein isoform 2 antibody
    • Brevican proteoglycan antibody
    • Chondroitin sulfate proteoglycan 7 antibody
    • Chondroitin sulfate proteoglycan BEHAB antibody
    • CSPG7 antibody
    • MGC13038 antibody
    see all

图片

  • Western blot - Anti-Brevican antibody (ab111719)
    Western blot - Anti-Brevican antibody (ab111719)
    Anti-Brevican antibody (ab111719) at 1/500 dilution + MCF-7 whole cell lysate
    at 30 µg

    Predicted band size: 99 kDa



    7.5% SDS-PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody (ab111719)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody (ab111719)
    ab111719 at 1/500 dilution staining Brevican in Formalin-fixed, paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

文献 (5)

发表研究结果有使用 ab111719？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab111719 被引用在 5 文献中.

  • Strokotova AV  et al. Prolonged use of temozolomide leads to increased anxiety and decreased content of aggrecan and chondroitin sulfate in brain tissues of aged rats. Biomed Rep 20:7 (2024). PubMed: 38124768
  • Governa V  et al. Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35217608
  • Park SS  et al. Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex. BMC Neurosci 21:16 (2020). PubMed: 32334536
  • Felemban M  et al. Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. Acta Biomater 74:207-221 (2018). PubMed: 29777959
  • Orlando C  et al. Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner. J Neurosci 32:18009-17, 18017a (2012). ICC/IF ; Mouse . PubMed: 23238717

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Brevican antibody

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (human astrocytes)
Specification
human astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 05 2012

Question

ReviewID: xxx AbID: 111719, Anti-Brevican antibody Rating: Inconclusive Image: Anti-Brevican antibody for Immunocytochemistry/ Immunofluorescence (Human) Sample: Species: Human Type: Cultured Cells Specification: human astrocytes Application: Application: Fixative: Paraformaldehyde Permeabilization: Yes Blocking step: Serum as blocking agent for 1 hour at 25°C Other detail: Dilution: 1/50 Incubation time: 12 hours at 4°C Secondary Antibody: Name: Non-Abcam Antibody was used: Alexa Fuor 488 goat anti-rabbit IgG (other company) Conjugation: Alexa Fluor® 488 Dilution: 1/1000 Additional Data: Additional Notes: Brevican staining of (A) human-derived NSCs and (B) human astrocytes. We have tried this antibody with the following protocols first we fixed the cells using 2% paraformaldehyde for 15 minutes followed by blocking in PBS containing 0.3% triton, 10% goat serum and 10% BSA for one hour. Then incubated the cells with the blocking buffer with antibody at 1:50, 1:250 , 1:500 and 1:1000 dilutions o/n at 4C. We also repeated the above procedure with 20 minutes fixation and also in another trial used 2%formaldehyde instead of paraformaldehyde and fixed the cells for 10 minutes. Despite the fact that we dont know if this antibody is made agaist an extracellular or intracelluar epitopes, we decided to try live staining with Bcan antibody at the different dilution at 37C for one hour. In conclusion, we could not detect a specific staining of brevican.

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Answer

Thank you for submitting an Abreview for ab111719. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
- try another fixation method (e.g. methanol or acetone)
- do a no primary control to check how much background comes from the secondary antibody alone
- I can't tell for sure if a confocal microscope was used for your images. If not, it might be worthwhile trying, since you would be able to move the focal plane through the cell and could be able to see the extracellular staining better and distinguish between cytoplasmic background and membrane/ECM staining.

If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

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Question

Phoned to ask if this product recognizes the extracellular region of Brevican.

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Answer

Thank you for contacting us.

Do you happen to know which region of Brevican is membrane bound?The immunogen for ab111719 falls within the region betweenamino acids 83 and 262 of human Brevican. According to the SwissProt entry for this protein (Q96GW7), this region encompasses a portion of the Ig-like V-type domain, the entire link 1 domain and a portion of the link 2 domain. However, I am unsure if any of these domains are involved in membrane binding.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Test ab111719 in ICC/IF.

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Answer

DISCOUNT CODE: *****
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab111719 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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