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Cell Biology Cell Cycle Markers

Anti-BrdU抗体- Proliferation Marker (ab1893)

  • Datasheet
  • SDS
Reviews (16)Q&A (7)References (151)

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Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)

Key features and details

  • Sheep polyclonal to BrdU - Proliferation Marker
  • Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG

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概述

  • 产品名称

    Anti-BrdU抗体- Proliferation Marker
    参阅全部 BrdU 一抗
  • 描述

    羊多克隆抗体to BrdU - Proliferation Marker
  • 宿主

    Sheep
  • 经测试应用

    适用于: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WBmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to BrdU. Coupled to HGG (Human Gamma Globulin).

  • 常规说明

    Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.60
    Constituent: 0.4% PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

相关产品

  • Isotype control

    • Sheep IgG - Isotype Control (ab37385)
  • Related Products

    • BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1893于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-FrFl (1)
Use at an assay dependent concentration.
IHC-P (8)
Use a concentration of 10 µg/ml.
IHC-Fr (2)
Use a concentration of 10 µg/ml.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
IHC-FrFl
Use at an assay dependent concentration.
IHC-P
Use a concentration of 10 µg/ml.
IHC-Fr
Use a concentration of 10 µg/ml.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 相关性

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 细胞定位

    Nuclear
  • 别名

    • Bromodeoxyuridine antibody
    • BUdr antibody

图片

  • Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)
    Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.

    (Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreview

    ab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

实验方案

  • Recommended protocol for BrdU staining with ab1893

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (151)

发表研究结果有使用 ab1893?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1893 被引用在 151 文献中.

  • Shimizu Y & Kawasaki T Histone acetyltransferase EP300 regulates the proliferation and differentiation of neural stem cells during adult neurogenesis and regenerative neurogenesis in the zebrafish optic tectum. Neurosci Lett 756:135978 (2021). PubMed: 34023416
  • Recabal A  et al. The FGF2-induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic-dependent pathway. J Neurochem 156:182-199 (2021). PubMed: 32936929
  • Jang H  et al. FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress. Cell Death Dis 12:407 (2021). PubMed: 33859174
  • Li B  et al. Apelin/APJ relieve diabetic cardiomyopathy by reducing microvascular dysfunction. J Endocrinol 249:1-18 (2021). PubMed: 33504680
  • Vinsland E  et al. The zinc finger/RING domain protein Unkempt regulates cognitive flexibility. Sci Rep 11:16299 (2021). PubMed: 34381067
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 23 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic mouse spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x DAKO antigen retrieval buffer
Permeabilization
Yes - 0.3% Triton X-100 in PBS for 5 minutes
Specification
embryonic mouse spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Laura Lahti

Verified customer

提交于 Dec 04 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain SVZ)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - tritonX 0.05%
Specification
Brain SVZ
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 15 2018

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody - Proliferation Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBS + 0.2% Triton X-100 + 4% BSA
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alexandre Magno

Verified customer

提交于 Aug 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
liver
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 27 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (adrenal gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
adrenal gland
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 21 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ramos cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization
No
Specification
Ramos cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 25 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 25 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody - Proliferation Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cultured Cells (COS7)
Specification
COS7
Permeabilization
Yes - 0.5% TritonX100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 27 2014

Question

I see this kit has not been tested yet against IDU, do you have any testing data for FDU cross-reactivity?

Read More

Abcam community

Verified customer

Asked on Mar 11 2013

Answer

Unfortunately we do not have any cross-reactivity data against FDU.

Read More

Abcam Scientific Support

回复于 Mar 11 2013

Question

The protocol with this kit states to use borate buffer, point 5 of the protocol.
We do not have this buffer in our lab. Is there any other buffer we can use? Or do you have the recipe for this one?

'Immediately after the acid washes Borate buffer (0.1M) is then used to buffer the cells for 12 minutes at r.t'
How do we neutralize this afterwards?

Read More

Abcam community

Verified customer

Asked on Nov 30 2012

Answer

Use 0.1M Borate buffer. Recipe is:

Sodium Tetraborate (fw =381.4) --> 38.14 grams to 1000 ml water, pH 8.5

After the soaking in the Borate buffer (pH 8.5) they are neutralized by rinsing in the PBS buffer (pH 7.4), which is listed on both protocols

Read More

Abcam Scientific Support

回复于 Nov 30 2012

1-10 of 23 Abreviews or Q&A

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