Anti-BrdU抗体[IIB5] (ab8955)
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.cn/ps/products/8/ab8955/Images/ab8955-287553-anti-brdu-antibody-iib5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)")
Key features and details
- Mouse monoclonal [IIB5] to BrdU
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-BrdU抗体[IIB5]
参阅全部 BrdU 一抗 -
描述
小鼠单克隆抗体[IIB5] to BrdU -
宿主
Mouse -
特异性
This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU). -
经测试应用
适用于: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to BrdU conjugated to bovine serum albumin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
IIB5 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr |
1/100 - 1/200.
ABC method. |
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| IHC-P | (1) |
1/100 - 1/200.
ABC method. |
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
1/100 - 1/200. ABC method. |
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IHC-P
1/100 - 1/200. ABC method. |
靶标
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相关性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
细胞定位
Nuclear -
别名
- Bromodeoxyuridine antibody
- BUdr antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.cn/ps/products/8/ab8955/Images/ab8955-301028-anti-brdu-antibody-iib5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.
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![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.cn/ps/products/8/ab8955/Images/ab8955-391620-anti-brdu-antibody-iib5-immunocytochemistry-mr65.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.cn/ps/products/8/ab8955/Images/ab8955-301027-anti-brdu-antibody-iib5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.
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![Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.cn/ps/products/8/ab8955/Images/ab8955-301026-anti-brdu-antibody-iib5-flow-cytometry.jpg)
Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (30)
ab8955 被引用在 30 文献中.
- Liang J et al. Minocycline Attenuates Sevoflurane-Induced Postoperative Cognitive Dysfunction in Aged Mice by Suppressing Hippocampal Apoptosis and the Notch Signaling Pathway-Mediated Neuroinflammation. Brain Sci 13:N/A (2023). PubMed: 36979321
- Llorente A et al. MAF amplification licenses ERα through epigenetic remodelling to drive breast cancer metastasis. Nat Cell Biol 25:1833-1847 (2023). PubMed: 37945904
- Ding Y et al. Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model. Biol Res 56:65 (2023). PubMed: 38041203
- Naveed A et al. NEAT1 polyA-modulating antisense oligonucleotides reveal opposing functions for both long non-coding RNA isoforms in neuroblastoma. Cell Mol Life Sci 78:2213-2230 (2021). PubMed: 32914209
- Rozen-Gagnon K et al. Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses. Cell Host Microbe 29:834-848.e13 (2021). PubMed: 33794184