Anti-BrdU抗体[IIB5] (ab8152)
Key features and details
- Mouse monoclonal [IIB5] to BrdU
- Suitable for: IHC-FoFr, ICC/IF, Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-BrdU抗体[IIB5]
参阅全部 BrdU 一抗 -
描述
小鼠单克隆抗体[IIB5] to BrdU -
宿主
Mouse -
特异性
BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies. -
经测试应用
适用于: IHC-FoFr, ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to BrdU conjugated to bovine serum albumin.
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阳性对照
- Bromodeoxyuridine labeled cells.
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常规说明
The following product is available as purified antibody (purified by affinity chromatography) together with several conjugated versions:
Anti-BrdU antibody [BU1/75 (ICR1)] (ab6326)
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
IIB5 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Buffer
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Related Products
应用
应用 | Ab评论 | 说明 |
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
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ICC/IF | (4) |
Use at an assay dependent concentration.
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Flow Cyt |
1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr |
1/5 - 1/10.
Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide. |
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IHC-P | (2) |
1/5 - 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Enzymatic antigen retrieval with proteases can also be used. |
说明 |
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IHC-FoFr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/5 - 1/10. Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide. |
IHC-P
1/5 - 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Enzymatic antigen retrieval with proteases can also be used. |
靶标
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相关性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
细胞定位
Nuclear -
别名
- Bromodeoxyuridine antibody
- BUdr antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8152)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab8152 (1/100) staining BrdU in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further esperimental details please see Abreview.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8152)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of rat brain tissue, staining BrdU with ab8152.
Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/20 in diluent) for 18 hours at 25°C. A Cy3®-conjugated donkey anti-mouse polyclonal IgG was used as the secondary antibody. -
Cells were pulse labeled with 10 µM BrdU for 30 min, rinsed twice in prewarmed PBS, and chased in prewarmed culture medium, supplemented with 5 mM deoxythymidine. Incorporated BrdU was detected after ethanol fixation of the cells, which were than rinsed once in PBS and resuspended in 2 ml of 0.4 mg/ml pepsin in 0.1 N HCl. After 30 min at room temperature cells were pelleted, resuspended in 2 N HCl, and incubated for another 30 min at 37°C. Cells were rinsed in 0.1 M borate buffer, pH 8.5, and PBS/BSA (1 mg/ml BSA in PBS). Appropriately diluted mouse anti-BrdU antibody (clone IIB5) was added to the cell pellet, resuspended in 100 micro liters PBS/BSA. After incubation for 1 h at room temperature, the cells were rinsed twice in PBS/BSA. For visualization, FITC-conjugated Fab2 fragments of rabbit anti-mouse Ig antibody were added in a 1/10 dilution. After incubation for 45 min at room temperature samples were rinsed twice in PBS/BSA and the cells were finally resuspended in 0.5 ml cold PBS supplemented with 100 microgram/ml RNAse and 20 µg/mL propidium iodide. The samples were allowed to stand for 15 min on ice in the dark before flow cytometric analysis. In the negative control the primary antibody was omitted.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (67)
ab8152 被引用在 67 文献中.
- Dubois-Pot-Schneider H et al. Transcriptional and Epigenetic Consequences of DMSO Treatment on HepaRG Cells. Cells 11:N/A (2022). PubMed: 35892596
- Ghosh B et al. Loss of E-cadherin is causal to pathologic changes in chronic lung disease. Commun Biol 5:1149 (2022). PubMed: 36309587
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Vlach M et al. Liposome-Mediated Gene Transfer in Differentiated HepaRG™ Cells: Expression of Liver Specific Functions and Application to the Cytochrome P450 2D6 Expression. Cells 11:N/A (2022). PubMed: 36497165
- Hardy RA et al. Role of age and neuroinflammation in the mechanism of cognitive deficits in sickle cell disease. Exp Biol Med (Maywood) 246:106-120 (2021). PubMed: 32962408