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重组

重组Anti-Brd4抗体[BL-149-2H5] - BSA free (ab272042)

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Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free
  • Suitable for: WB, IP, ChIP-sequencing, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Brd4抗体[BL-149-2H5] - BSA free
    参阅全部 Brd4 一抗

  • 描述

    兔单克隆抗体[BL-149-2H5] to Brd4 - BSA free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, ChIP-sequencing, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide within Human Brd4 aa 1312-1362. The exact sequence is proprietary. NP_490597.1 and Gene ID 23476.
    Database link: O60885

  • 阳性对照

    • WB: HeLa, HEK293T, Jurkat, MDA-MB-231, A-549, SW620, SK-MEL-28, BW5147.3 whole cell lysate. IP: HeLa whole cell lysate. ChIP: Mia PaCa-2 cells. ICC/IF: HeLa cells.

  • 常规说明

    ab272042 is the BSA-free version of ab243862.

    This product is sold under License from Bethyl Laboratories, Inc.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272042于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ChIP-sequencing
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ChIP-sequencing
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Plays a role in a process governing chromosomal dynamics during mitosis.

  • 组织特异性

    Ubiquitously expressed.

  • 疾病相关

    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.

  • 序列相似性

    Contains 2 bromo domains.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Brd4 antibody
      • BRD4-NUT FUSION antibody
      • BRD4-NUT fusion oncoprotein antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Mitotic chromosome-associated protein antibody
      • Protein HUNK1 antibody
      see all

    图片

    • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      All lanes : Anti-Brd4 antibody [BL-149-2H5] (ab243862) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HEK293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : MDA-MB-231 whole cell lysate
      Lane 5 : A-549 whole cell lysate
      Lane 6 : SW620 whole cell lysate
      Lane 7 : SK-MEL-28 whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG

      Exposure time: 3 seconds


      Detection: Chemiluminescence.

      Lysates prepared using NETN lysis buffer.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

      brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with ab243862 at 20 µL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.

      Lane 1: ab243862 IP in HeLa cell lysate.

      Lane 2: Contol IgG in HeLa cell lysate.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Western blot - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Anti-Brd4 antibody [BL-149-2H5] (ab243862) at 1/1000 dilution + BW5147.3 (Mouse T cell lymphoma cell line from thymus) whole cell lysate at 50 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG


      Chemiluminescence with an exposure time of 3 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272042？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272042 尚未被引用在任何文献中。

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