Anti-Brd4抗体(ab223223)
Key features and details
- Goat polyclonal to Brd4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Brd4抗体
参阅全部 Brd4 一抗 -
描述
山羊多克隆抗体to Brd4 -
宿主
Goat -
特异性
ab223223 is expected to recognize both reported isoforms (NP_490597.1; NP_055114.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Dog, Human
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免疫原
Synthetic peptide corresponding to Human Brd4 aa 1-100 (internal sequence) (Cysteine residue). (NP_490597.1).
Database link: O60885 -
阳性对照
- WB: Mouse testis lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab223223 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223223于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 152 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 152 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Plays a role in a process governing chromosomal dynamics during mitosis. -
组织特异性
Ubiquitously expressed. -
疾病相关
Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein. -
序列相似性
Contains 2 bromo domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 484869 Dog
- Entrez Gene: 23476 Human
- Entrez Gene: 57261 Mouse
- Entrez Gene: 362844 Rat
- Omim: 608749 Human
- SwissProt: O60885 Human
- SwissProt: Q9ESU6 Mouse
- Unigene: 187763 Human
see all -
别名
- Brd4 antibody
- BRD4-NUT FUSION antibody
- BRD4-NUT fusion oncoprotein antibody
see all
图片
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Western blot - Anti-Brd4 antibody (ab223223)Anti-Brd4 antibody (ab223223) at 1 µg/ml + Mouse testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (0)
ab223223 尚未被引用在任何文献中。
