Name: Anti-BRD1/BRL antibody [EPR12960]
Brand: RabMAb
SKU: ab181060
Rating: 5 (2 reviews)

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12960] to BRD1/BRL
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-BRD1/BRL抗体[EPR12960]
参阅全部 BRD1/BRL 一抗
描述

兔单克隆抗体[EPR12960] to BRD1/BRL
宿主

Rabbit
经测试应用

适用于: WB, IHC-P, IPmore details
不适用于: ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: K-562 and Raji cell lysate; IHC-P: Human testis and lung carcinoma tissue, Mouse testis tissue and Rat testis tissue. IP: K-562 whole cell lysate, Mouse testis tissue lysate
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR12960
同种型

IgG
研究领域
- Epigenetics and Nuclear Signaling
- Bromodomains

Alternative Versions
- Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Raji whole cell lysate (ab30124)
Recombinant Protein
- Recombinant Human BRD1/BRL protein (ab187427)

The Abpromise guarantee

Abpromise™承诺保证使用ab181060于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(1)	1/1000 - 1/2000. Predicted molecular weight: 120 kDa.
IHC-P		1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

说明
WB 1/1000 - 1/2000. Predicted molecular weight: 120 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

应用说明

Is unsuitable for ICC/IF.

功能

Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
组织特异性

Highly expressed in testis.
序列相似性

Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
细胞定位

Nucleus.
Target information above from: UniProt accession O95696 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 23774 Human
- Entrez Gene: 223770 Mouse
- Entrez Gene: 315210 Rat
- Omim: 604589 Human
- SwissProt: O95696 Human
- Unigene: 127950 Human
别名
- BR140-like protein antibody
- BRD1 antibody
- BRD1_HUMAN antibody
- BRL antibody
- Bromodomain and PHD finger-containing protein 2 antibody
- Bromodomain containing 1 antibody
- Bromodomain-containing protein 1 antibody
- BRPF2 antibody
- DKFZp686F0325 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Western blot - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

All lanes : Anti-BRD1/BRL antibody [EPR12960] (ab181060) at 1/2000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.

Lane 1 (input): K-562 whole cell lysate (10µg)

Lane 2 (+): ab181060 + K-562 whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in K-562 whole cell lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in mouse testis tissue lysate.

Lane 1 (input): Mouse testis tissue lysate (10µg)

Lane 2 (+): ab181060 + Mouse testis tissue lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in mouse testis tissue lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human lung carcinoma. The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 10602503). The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab181060？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab181060 被引用在 2 文献中.

Li Z et al. Expression Characteristics and Clinical Correlations of BRD1 in Colorectal Cancer Samples. Technol Cancer Res Treat 20:15330338211039678 (2021). PubMed: 34482774
Kulej K et al. Time-resolved Global and Chromatin Proteomics during Herpes Simplex Virus Type 1 (HSV-1) Infection. Mol Cell Proteomics 16:S92-S107 (2017). PubMed: 28179408

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Breast and Endometrial cell lines)

Gel Running Conditions

Reduced Denaturing (4-12% bis tris)

Loading amount

3 µg

Treatment

siCON or si-BRD1 smartpool for 24 hours

Specification

Breast and Endometrial cell lines

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katie Lynch

Verified customer

提交于 Dec 31 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Ovary)

Permeabilization

Yes - 0.5% Triton

Specification

Ovary

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katie Lynch

Verified customer

提交于 Dec 29 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (2)

客户评价及客户问答

Western blot abreview for Anti-BRD1/BRL antibody [EPR12960]

Immunocytochemistry/ Immunofluorescence abreview for Anti-BRD1/BRL antibody [EPR12960]