Anti-BRD1/BRL抗体(ab71877)
Key features and details
- Rabbit polyclonal to BRD1/BRL + Peregrin/BRPF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BRD1/BRL抗体 -
描述
兔多克隆抗体to BRD1/BRL + Peregrin/BRPF1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | |
| ICC/IF |
| 说明 |
|---|
应用说明
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 137 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 137 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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细胞定位
BRD1/BRL: Nucleus. Peregrin/BRPF1: Nucleus. Cytoplasm. Localization to the nucleus depends on KAT6A, ING5 and MEAF6. -
数据库链接
- Entrez Gene: 417731 Chicken
- Entrez Gene: 427590 Chicken
- Entrez Gene: 23774 Human
- Entrez Gene: 7862 Human
- Entrez Gene: 223770 Mouse
- Entrez Gene: 78783 Mouse
- Omim: 602410 Human
- Omim: 604589 Human
see all
图片
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Western blot - Anti-BRD1/BRL + Peregrin/BRPF1 antibody (ab71877)All lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 :Raji whole cell lysate (ab30124) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-BRD1/BRL + Peregrin/BRPF1 antibody (ab71877)ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml. -
Western blot - Anti-BRD1/BRL + Peregrin/BRPF1 antibody (ab71877)This image is a courtesy of Antibody Solutions LtdAll lanes : Anti-BRD1/BRL antibody (ab71877) at 1 µg/ml
Lane 1 : Lysates prepared from Mouse testis tissue.
Lane 2 : Lysates prepared from Mouse Lung tissue.
Lane 3 : Lysates prepared from Mouse Kidney tissue.
Lane 4 : Lysates prepared from GC-1 cell line.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 92 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 7 minutesGC-1 - mouse spermatogonia cell line
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab71877 被引用在 2 文献中.
- Wu Q et al. A chemical toolbox for the study of bromodomains and epigenetic signaling. Nat Commun 10:1915 (2019). PubMed: 31015424
- Kang H et al. Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in Drosophila. Genes Dev 31:1988-2002 (2017). PubMed: 29070704