重组Anti-BRCC36抗体[EPR4365] (ab108295)
![Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)](https://www.abcam.cn/ps/products/108/ab108295/Images/ab108295-280816-anti-brcc36-antibody-epr4365-western-blot.jpg "Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4365] to BRCC36
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BRCC36抗体[EPR4365]
参阅全部 BRCC36 一抗 -
描述
兔单克隆抗体[EPR4365] to BRCC36 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HAP1, MCF7, SKBR3, 293T, Human fetal kidney and HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4365 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108295于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
靶标
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功能
Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). -
组织特异性
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors. -
疾病相关
A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. -
序列相似性
Belongs to the peptidase M67A family. BRCC36 subfamily.
Contains 1 MPN (JAB/Mov34) domain. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690). - Information by UniProt
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数据库链接
- Entrez Gene: 79184 Human
- Entrez Gene: 210766 Mouse
- Entrez Gene: 316794 Rat
- Omim: 300617 Human
- SwissProt: P46736 Human
- SwissProt: P46737 Mouse
- SwissProt: B2RYM5 Rat
- Unigene: 558537 Human
see all -
别名
- BRCA1-A complex subunit BRCC36 antibody
- BRCA1/BRCA2 containing complex subunit 3 antibody
- BRCA1/BRCA2 containing complex subunit 36 antibody
see all
图片
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Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: Empty Lane
Lane 3: BRCC36 knockout HAP1 whole cell lysate (40 µg)
Lane 4: MCF7 whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108295 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108295 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. Ab108295 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)](https://www.abcam.cn/ps/products/108/ab108295/Images/ab108295-120803-anti-brcc36-antibody-epr4365-western-blot.jpg)
Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)All lanes : Anti-BRCC36 antibody [EPR4365] (ab108295) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : SKBR3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa -
![Anti-BRCC36 antibody [EPR4365] (ab108295)](https://www.abcam.cn/ps/products/108/ab108295/Images/ab108295-3-benefits-of-recombinant-antibodies.png)
Anti-BRCC36 antibody [EPR4365] (ab108295)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108295 被引用在 1 文献中.
- Jones MJK et al. Human DDK rescues stalled forks and counteracts checkpoint inhibition at unfired origins to complete DNA replication. Mol Cell 81:426-441.e8 (2021). PubMed: 33545059