AB108295

重组Anti-BRCC36抗体[EPR4365]

Anti-BRCC36 antibody [EPR4365]

RabMAb
Recombinant
KO Validated
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal BRCC36 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

查看别名

BRCC36, C6.1A, CXorf53, BRCC3, Lys-63-specific deubiquitinase BRCC36, BRCA1-A complex subunit BRCC36, BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRISC complex subunit BRCC36

2 Images

Lab

Western blot - Anti-BRCC36 antibody [EPR4365] (AB108295)

Lane 1 : Wild type HAP1 whole cell lysate (40 μg)
Lane 2 : Empty Lane
Lane 3 : BRCC36 knockout HAP1 whole cell lysate (40 μg)
Lane 4 : MCF7 whole cell lysate (40 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108295 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab108295 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. ab108295 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)

Predicted band size: 36 kDa

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Unknown

Western blot - Anti-BRCC36 antibody [EPR4365] (AB108295)

All lanes:

Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

SKBR3 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Predicted band size: 36 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4365

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% EDTA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed : 19214193, PubMed : 20656690, PubMed : 24075985, PubMed : 26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains (PubMed : 19214193, PubMed : 20656690, PubMed : 24075985, PubMed : 26344097). Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) (PubMed : 14636569, PubMed : 16707425, PubMed : 17525341, PubMed : 19202061, PubMed : 19261746, PubMed : 19261748, PubMed : 19261749). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed : 20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed : 20656690, PubMed : 24075985, PubMed : 26195665, PubMed : 26344097). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed : 19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed : 26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed : 24075985, PubMed : 26344097). Acts as a regulator of the NLRP3 inflammasome by mediating deubiquitination of NLRP3, leading to NLRP3 inflammasome assembly (By similarity). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed : 24075985). Deubiquitinates HDAC1 and PWWP2B leading to their stabilization (By similarity).

See full target information BRCC3

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadr6140 PubMed39742475

2025

Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skin.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly N Messingham,Paige T Richards,Anthony Fleck,Radhika A Patel,Marija Djurkovic,Jonah Elliff,Samuel Connell,Tyler P Crowe,Juan Munoz Gonzalez,Francoise Gourronc,Jacob A Dillard,Robert A Davey,Aloysius Klingelhutz,Olena Shtanko,Wendy Maury

Molecular cell 81:426-441.e8 PubMed33545059

2021

Human DDK rescues stalled forks and counteracts checkpoint inhibition at unfired origins to complete DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Mathew J K Jones,Camille Gelot,Stephanie Munk,Amnon Koren,Yoshitaka Kawasoe,Kelly A George,Ruth E Santos,Jesper V Olsen,Steven A McCarroll,Mark G Frattini,Tatsuro S Takahashi,Prasad V Jallepalli

The Journal of biological chemistry 290:5749-58 PubMed25568318

2015

Dexamethasone promotes hypertension by allele-specific regulation of the human angiotensinogen gene.

Applications

Unspecified application

Species

Unspecified reactive species

Varunkumar G Pandey,Sudhir Jain,Anita Rana,Nitin Puri,Sri Krishna C Arudra,Brahmaraju Mopidevi,Meenakshi Kaw,Alberto Nasjletti,Ashok Kumar

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-BRCC36抗体[EPR4365]

Anti-BRCC36 antibody [EPR4365]

Western blot - Anti-BRCC36 antibody [EPR4365] (AB108295)

Western blot - Anti-BRCC36 antibody [EPR4365] (AB108295)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原