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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation

Anti-BRCA1 (phospho S1423)抗体(ab2838)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Western blot - Anti-BRCA1 (phospho S1423) antibody (ab2838)

    Key features and details

    • Rabbit polyclonal to BRCA1 (phospho S1423)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-BRCA1 (phospho S1423)抗体
      参阅全部 BRCA1 一抗
    • 描述

      兔多克隆抗体to BRCA1 (phospho S1423)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
    • 免疫原

      Synthetic peptide corresponding to Human BRCA1 (phospho S1423). Synthetic phospho peptide (Human), which represents a portion of human Breast Cancer Gene 1 around serine 1423.
      Database link: P38398

    • 阳性对照

      • HeLa whole cell lysate treated with IR
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Ubiquitylation
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • BRCA1
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin E3 Enzymes
      • RING Finger E3 Ligase
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human BRCA1 protein (ab82204)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2838于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
    说明
    WB
    1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).

    靶标

    • 功能

      E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks.
    • 组织特异性

      Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
    • 通路

      Protein modification; protein ubiquitination.
    • 疾病相关

      Defects in BRCA1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.
      Defects in BRCA1 are a cause of susceptibility to breast-ovarian cancer familial type 1 (BROVCA1) [MIM:604370]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Note=Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer.
      Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].
    • 序列相似性

      Contains 2 BRCT domains.
      Contains 1 RING-type zinc finger.
    • 结构域

      The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.
      The RING-type zinc finger domain interacts with BAP1.
    • 翻译后修饰

      Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
      Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
    • 细胞定位

      Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.
    • Target information above from: UniProt accession P38398 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 672 Human
      • Entrez Gene: 712634 Rhesus monkey
      • Omim: 113705 Human
      • SwissProt: Q9GKK8 Chimpanzee
      • SwissProt: Q6J6I8 Gorilla
      • SwissProt: P38398 Human
      • SwissProt: Q6J6I9 Rhesus monkey
      • Unigene: 194143 Human
      • 别名

        • BRCA 1 antibody
        • BRCA1 antibody
        • BRCA1 DNA repair associated antibody
        • BRCA1/BRCA2 containing complex subunit 1 antibody
        • BRCA1/BRCA2-containing complex, subunit 1 antibody
        • BRCA1_HUMAN antibody
        • BRCAI antibody
        • BRCC 1 antibody
        • BRCC1 antibody
        • Breast and ovarian cancer susceptibility protein 1 antibody
        • Breast Cancer 1 antibody
        • Breast Cancer 1 Early Onset antibody
        • Breast cancer type 1 susceptibility protein antibody
        • BROVCA1 antibody
        • FANCS antibody
        • IRIS antibody
        • PNCA4 antibody
        • PPP1R53 antibody
        • Protein phosphatase 1 regulatory subunit 53 antibody
        • PSCP antibody
        • RING finger protein 53 antibody
        • RNF53 antibody
        see all

      图片

      • Western blot - Anti-BRCA1 (phospho S1423) antibody (ab2838)
        Western blot - Anti-BRCA1 (phospho S1423) antibody (ab2838)
        ab2838 at a 1:2500 dilution staining phospho BRCA 1 (Ser 1423) in HeLa whole cell lysate (25µg / lane, +/- IR) by Western blot (ECL). ab2838 at a 1:2500 dilution staining phospho BRCA 1 (Ser 1423) in HeLa whole cell lysate (25µg / lane, +/- IR) by Western blot (ECL).

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab2838?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2838 被引用在 2 文献中.

      • Gonzalez ME  et al. Histone methyltransferase EZH2 induces Akt-dependent genomic instability and BRCA1 inhibition in breast cancer. Cancer Res 71:2360-70 (2011). WB ; Human . PubMed: 21406404
      • Gonzalez ME  et al. Downregulation of EZH2 decreases growth of estrogen receptor-negative invasive breast carcinoma and requires BRCA1. Oncogene 28:843-53 (2009). WB ; Human . PubMed: 19079346

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