Anti-BRAG2抗体(ab122442)
Key features and details
- Rabbit polyclonal to BRAG2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BRAG2抗体
参阅全部 BRAG2 一抗 -
描述
兔多克隆抗体to BRAG2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human BRAG2 aa 28-96 (N terminal).
Sequence:PSSETGTSLDSPSAYPQGPLVPGSSLSPDHYEHTSVGAYGLYSGPPGQQQ RTRRPKLQHSTSILRKQAE
Database link: Q6DN90 -
阳性对照
- Human skin tissue; Human cell line A431
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122442于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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功能
In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. -
组织特异性
Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis. -
序列相似性
Belongs to the BRAG family.
Contains 1 IQ domain.
Contains 1 PH domain.
Contains 1 SEC7 domain. -
细胞定位
Cytoplasm. Nucleus. At steady state, may be preferentially cytosolic. - Information by UniProt
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数据库链接
- Entrez Gene: 9922 Human
- Omim: 610166 Human
- SwissProt: Q6DN90 Human
- Unigene: 475506 Human
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别名
- ADP-ribosylation factors guanine nucleotide-exchange protein 100 antibody
- ADP-ribosylation factors guanine nucleotide-exchange protein 2 antibody
- ARF GEP100 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAG2 antibody (ab122442)
ab122442, at a 1/50 dilution, staining BRAG2 in paraffin embedded Human skin tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-BRAG2 antibody (ab122442)ab122442, at 4 µg/ml, staining BRAG2 in Human A431 cell line, in green, by Immunoflouroscence.
Fixation/Permeabilization: PFA/Triton X-100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122442 被引用在 1 文献中.
- Humphreys D et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). WB ; Human coronavirus . PubMed: 24085844