Anti-Brachyury / Bry抗体(ab20680)
Key features and details
- Rabbit polyclonal to Brachyury / Bry
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Brachyury / Bry抗体
参阅全部 Brachyury / Bry 一抗 -
描述
兔多克隆抗体to Brachyury / Bry -
宿主
Rabbit -
特异性
The immunogen used to raise this antibody has 81% homology with the Mouse Brachyury protein. Some customers have successfully used ab20680 on mouse samples, however we have not been successful detecting Brachyury in this species in our own testing and therefore cannot guarantee mouse reactivity. Please contact Abcam Scientific Support for more information.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Dog
不与反应:
Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20680于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. -
疾病相关
Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication. -
序列相似性
Contains 1 T-box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6862 Human
- Omim: 601397 Human
- SwissProt: O15178 Human
- Unigene: 389457 Human
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别名
- BRAC_HUMAN antibody
- Brachyury homolog antibody
- Brachyury protein antibody
see all
图片
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Western blot - Anti-Brachyury / Bry antibody (ab20680)All lanes : Anti-Brachyury / Bry antibody (ab20680) at 1 µg/ml
Lane 1 : MUG-Chor1 (human sacral bone chordoma) at 10 µg
Lane 2 : MCF7 whole cell lysate (negative control) at 15 µg
Lane 3 : Human embryonic stem cells (pluripotent) (negative control) at 10 µg
Lane 4 : Human embryonic stem cells (mesoderm) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab20680 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab20680 被引用在 54 文献中.
- Huang S et al. In vivo development and single-cell transcriptome profiling of human brain organoids. Cell Prolif 55:e13201 (2022). PubMed: 35141969
- Tang PC et al. Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43. Stem Cell Res 61:102758 (2022). PubMed: 35364395
- Salerno AG et al. S-nitrosoglutathione reductase (GSNOR) deficiency accelerates cardiomyocyte differentiation of induced pluripotent stem cells. J Cardiovasc Aging 1:N/A (2021). PubMed: 34790975
- Ou M et al. Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes. Biol Open 10:N/A (2021). PubMed: 33589441
- Chen M et al. Transactivation of SOX5 by Brachyury promotes breast cancer bone metastasis. Carcinogenesis 41:551-560 (2020). PubMed: 31713604