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Anti-Bovine Papilloma Virus E2抗体[5H4] (ab1072)

Submit a review Q&A (1)
Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

    Key features and details

    • Mouse monoclonal [5H4] to Bovine Papilloma Virus E2
    • Suitable for: ICC/IF, ELISA, IP
    • Reacts with: Bovine papillomavirus
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Bovine Papilloma Virus E2抗体[5H4]
      参阅全部 Bovine Papilloma Virus E2 一抗

    • 描述

      小鼠单克隆抗体[5H4] to Bovine Papilloma Virus E2

    • 宿主

      Mouse

    • 经测试应用

      适用于: ICC/IF, ELISA, IPmore details
      不适用于: WB

    • 种属反应性

      与反应: Bovine papillomavirus

    • 免疫原

      Recombinant full length protein corresponding to Bovine Bovine Papilloma Virus E2. Recombinant full length BPV type 1 E2 protein purified from E. coli
      Database link: P03122

    • 表位

      Conformational

    • 阳性对照

      • ICC/IF: Human U2OS cells expressing BPV E2 protein

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1072于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    Use a concentration of 1 - 2 µg/ml.
    ELISA
    Use at an assay dependent dilution.
    IP
    Use at an assay dependent dilution.
    说明
    ICC/IF
    Use a concentration of 1 - 2 µg/ml.
    ELISA
    Use at an assay dependent dilution.
    IP
    Use at an assay dependent dilution.
    应用说明
    Is unsuitable for WB.

    靶标

    • 相关性

      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.

    • 细胞定位

      Host cell nucleus

    • 数据库链接

      • 别名

        • BPVgp4 antibody
        • E2 antibody
        • Regulatory protein E2 antibody

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
        Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

        Immunofluorescent staining of U2OS cells expressing BPV E2 protein with ab1072. The cells were counter-stained with DAPI, and the upper shows an image taken with illumination at both 460 nm and 488 nm, whereas the lower image shows illumination at 488 nm.

      文献 (0)

      发表研究结果有使用 ab1072？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab1072 尚未被引用在任何文献中。

      客户评价及客户问答

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      Question

      BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM multiple nonspecific bands with background. no specific bands revealed SAMPLE cell extracts from transient transfected 293T cells. E2-p300 fusion expression plasmids were transfected superfect was used as transfect reagents PRIMARY ANTIBODY E2 antibody (1:1000) ab1072, fresh shipment DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED positive control: 1) same cells were transfected with cyclin D1 and subsequent western blot was beautiful; 2)same cell lysate was bloted with another antibody, (anti-GDI), the results was nice and clean; our conclusion is that the E2 primary antibody does not work! ANTIBODY STORAGE CONDITIONS 4C SAMPLE PREPARATION standard tris buffer plus np-40, pmsf, dtt and protease inhibitors(tablet) AMOUNT OF PROTEIN LOADED 60ug ELECTROPHORESIS/GEL CONDITIONS 7% sds-page TRANSFER AND BLOCKING CONDITIONS semitry transfer, 5% milk in tpbs SECONDARY ANTIBODY anti-mouse-HRP conjugated HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES We are well experienced lab with many publications each year,and western blot is a routine in our lab. we have applied positive controls(for transfection, primary antibody, and 2nd antibody) for the western blot. E2 antibody did not pick up any specific signal other than many nonspecific bands. please ship another antibody that DO works in your hands, or please refund us, so we could buy E2 antibody from other sources.We are addressing reviewer's question for a paper, please understand that we are underpressure and we need to get the E2 western blot to work within 2-3 weeks. thank you.

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      Answer

      I'm sorry to hear you are having problems with ab1072. I am currently trying to find out more information about the protocol used to test the antibody in WB. Have you tested the blot without milk blocking and if the non specific bands could be due to the secondary antibody? How long did you incubate the primary antibody for? The absence of a band could be due to the levels of Bovine Papilloma Virus E2 being below WB detection levels, I am trying to find a positive control to help you and I apologise for the delay and appreciate your patience,

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