Anti-Bovine Papilloma Virus E2抗体[1H10] (ab77174)
![Western blot - Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)](https://www.abcam.cn/ps/products/77/ab77174/Images/ab77174-172813-ab77174wb.jpg "Western blot - Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)")
Key features and details
- Mouse monoclonal [1H10] to Bovine Papilloma Virus E2
- Suitable for: WB, ICC/IF
- Reacts with: Bovine papillomavirus
- Isotype: IgG
概述
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产品名称
Anti-Bovine Papilloma Virus E2抗体[1H10]
参阅全部 Bovine Papilloma Virus E2 一抗 -
描述
小鼠单克隆抗体[1H10] to Bovine Papilloma Virus E2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Bovine papillomavirus -
免疫原
Recombinant full length Bovine Papillomavirus E2-protein expressed in E Coli.
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表位
Mapped to amino acids 208-218 (RPDGVWVASEG) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1H10 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa.
WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. |
|
| ICC/IF |
Use a concentration of 1 µg/ml.
1/1000 for 1 hour at RT, in PBS with 1% BSA. |
| 说明 |
|---|
|
WB
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa. WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. |
|
ICC/IF
Use a concentration of 1 µg/ml. 1/1000 for 1 hour at RT, in PBS with 1% BSA. |
靶标
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相关性
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
细胞定位
Host cell nucleus -
数据库链接
- SwissProt: P03122 Bovine papillomavirus
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别名
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
图片
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Western blot - Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)All lanes : Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)
Lane 1 : Recombinant purified Bovine Papillomavirus E2 protein at 10000 µg
Lane 2 : Recombinant purified Bovine Papillomavirus E2 protein at 5000 µg
Lane 3 : Recombinant purified Bovine Papillomavirus E2 protein at 1000 µg
Lane 4 : Recombinant purified Bovine Papillomavirus E2 protein at 500 µg
Predicted band size: 45 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab77174 被引用在 2 文献中.
- Kurg R et al. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers. J Virol 80:11218-25 (2006). WB . PubMed: 16943289
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). WB, ELISA . PubMed: 10233926