Anti-Bovine Papilloma Virus E2抗体[1E4] (ab980)
![Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)](https://www.abcam.cn/ps/products/0/ab980/Images/ab980-172809-anti-bovine-papilloma-virus-e2-antibody-1e4-western-blot.jpg "Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)")
Key features and details
- Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
- Suitable for: WB, IP, ICC/IF
- Reacts with: Bovine papillomavirus
- Isotype: IgG1
概述
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产品名称
Anti-Bovine Papilloma Virus E2抗体[1E4]
参阅全部 Bovine Papilloma Virus E2 一抗 -
描述
小鼠单克隆抗体[1E4] to Bovine Papilloma Virus E2 -
宿主
Mouse -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Bovine papillomavirus -
免疫原
Mapped to amino acids 250-280.
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表位
Not Determined. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1E4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
1hr, RT. Dilute in a diluent containing 2% non-fat dry milk in TBST. (Background can be reduced by adding NaCl up to a concentration of 1.5 M.) |
|
| IP |
Use at an assay dependent concentration.
To isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose. |
|
| ICC/IF |
1/500 - 1/1000.
1hr, RT. Dilute in a diluent containing 1% BSA in PBS. |
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 45 kDa. 1hr, RT. Dilute in a diluent containing 2% non-fat dry milk in TBST. (Background can be reduced by adding NaCl up to a concentration of 1.5 M.) |
|
IP
Use at an assay dependent concentration. To isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose. |
|
ICC/IF
1/500 - 1/1000. 1hr, RT. Dilute in a diluent containing 1% BSA in PBS. |
靶标
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相关性
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
细胞定位
Host cell nucleus -
数据库链接
- SwissProt: P03122 Bovine papillomavirus
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别名
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
图片
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Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg
Predicted band size: 45 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab980 被引用在 5 文献中.
- Garcia-Diaz B et al. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling. Acta Neuropathol 138:457-476 (2019). PubMed: 31011859
- Yamauchi T et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). PubMed: 28069738
- Bocco BM et al. Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice. Endocrinology 157:3682-95 (2016). PubMed: 27501182
- Della Gatta G et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . PubMed: 22366949
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926