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重组RabMAb

重组Anti-Bone Sialoprotein抗体[EPR23367-50] - BSA and Azide free (ab272320)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
  • Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
  • Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
  • Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23367-50] to Bone Sialoprotein - BSA and Azide free
  • Suitable for: IP, IHC-P, IHC-Fr
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Bone Sialoprotein抗体[EPR23367-50] - BSA and Azide free
    参阅全部 Bone Sialoprotein 一抗

  • 描述

    兔单克隆抗体[EPR23367-50] to Bone Sialoprotein - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IP, IHC-P, IHC-Frmore details
    不适用于: WB

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse E14.5 tissue. IHC-Fr: Mouse E14.5 tissue. IP: Mouse fetal bone tissue lysate.

  • 常规说明

    ab272320 is the carrier-free version of ab270457.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272320于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
说明
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
应用说明
Is unsuitable for WB.

靶标

  • 功能

    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

  • 翻译后修饰

    N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
    O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
    Sulfated on either Tyr-313 or Tyr-314.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • BNSP antibody
      • Bone sialoprotein 2 antibody
      • Bone sialoprotein II antibody
      • BSP antibody
      • BSP II antibody
      • BSPII antibody
      • Cell binding sialoprotein antibody
      • Cell-binding sialoprotein antibody
      • IBSP antibody
      • Integrin binding sialoprotein antibody
      • Integrin-binding sialoprotein antibody
      • SIAL_HUMAN antibody
      • SPII antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

      Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling Bone Sialoprotein with ab270457 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on bones of mouse E14.5 (PMID: 24927920). The section was incubated with ab270457 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270457).

    • Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
      Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

      Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-1-- permeabilized mouse E14.5 embryo tissue labeling Bone Sialoprotein with ab270457 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse embryonic bone. The nuclear counter stain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Secondary antibody is b150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270457).

    • Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
      Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

      Bone Sialoprotein was immunoprecipitated from 0.35 mg Mouse fetal bone tissue lysate using ab270457 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse fetal bone tissue lysate 10ug

      Lane 2: ab270457 IP in Mouse fetal bone tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270457 in Mouse fetal bone tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 41 sseconds.

      The molecular weight observed is consistent with literature (PMID: 14647232)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270457).

    • Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
      Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272320？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272320 尚未被引用在任何文献中。

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