重组Anti-Bok抗体[BOK-R1-5-1]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (AB233072)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Bok antibody [BOK-R1-5-1] (AB233072)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling Bok with ab233072 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cellsincubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-Bok antibody [BOK-R1-5-1] (AB233072)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator Dr. Thomas Kaufmann, University of Bern, Switzerland.

All lanes:

Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

Lane 1:

Wild-type MEF (Mouse embryonic fibroblast cell line) cell lysate at 20 µg

Lane 2:

Bok knockout MEF cell lysate at 20 µg

Lane 3:

Wild-type HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Lane 4:

Bok knockout HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-Bok antibody [BOK-R1-5-1] (AB233072)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-3 and 8 : 70 seconds; Lanes 4-5 : 125 seconds; Lanes 6-7 : 37 seconds; Lane 9 : 3 minutes.

The specific band for BOK is observed at 22 kDa; the other bands on the blots are non-specific.

Lanes 1 - 3:

Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/5000 dilution

Lanes 4 - 9:

Western blot - Anti-Bok antibody [BOK-R1-5-1] (ab233072) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Human fetal liver at 20 µg

Lane 3:

Human fetal kidney at 20 µg

Lane 4:

Mouse brain at 10 µg

Lane 5:

Mouse liver at 10 µg

Lane 6:

Rat brain at 10 µg

Lane 7:

Rat spleen at 10 µg

Lane 8:

C6 (rat glial tumor cell) whole cell lysate at 10 µg

Lane 9:

Human fetal brain at 10 µg

Secondary

Lanes 1 - 8:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 9:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 23 kDa

false