Anti-BOG抗体[OTI4E1] (ab118023)
![Western blot - Anti-BOG antibody [OTI4E1] (ab118023)](https://www.abcam.cn/ps/products/118/ab118023/Images/ab118023-129971-anti-bog-antibody-oti4e1-western-blot.jpg "Western blot - Anti-BOG antibody [OTI4E1] (ab118023)")
Key features and details
- Mouse monoclonal [OTI4E1] to BOG
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-BOG抗体[OTI4E1]
参阅全部 BOG 一抗 -
描述
小鼠单克隆抗体[OTI4E1] to BOG -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human BOG aa 1-200. Produced in HEK-293T cells (NP_006597).
Database link: O75884 -
阳性对照
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY BOG cDNA. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY BOG cDNA.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI4E1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118023于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 21 kDa.
|
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/500. Predicted molecular weight: 21 kDa. |
|
ICC/IF
1/100. |
靶标
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功能
May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. -
组织特异性
Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues. -
序列相似性
Belongs to the RBBP9 family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10741 Human
- Omim: 602908 Human
- SwissProt: O75884 Human
- Unigene: 69330 Human
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别名
- B5T overexpressed gene protein antibody
- B5T-overexpressed gene protein antibody
- BOG antibody
see all
图片
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Western blot - Anti-BOG antibody [OTI4E1] (ab118023)All lanes : Anti-BOG antibody [OTI4E1] (ab118023) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY RBBP9 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 21 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [OTI4E1] (ab118023)](https://www.abcam.cn/ps/products/118/ab118023/Images/ab118023-129970-anti-bog-antibody-oti4e1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [OTI4E1] (ab118023)pCMV6-ENTRY BOG cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for BOG using ab118023 at a 1/100 dilution in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab118023 尚未被引用在任何文献中。