AB249307

重组Anti-BOG抗体[EPR9905] - BSA and Azide free

Anti-BOG antibody [EPR9905] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal BOG antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

查看别名

BOG, RBBP10, RBBP9, Serine hydrolase RBBP9, B5T-overexpressed gene protein, Retinoblastoma-binding protein 10, Retinoblastoma-binding protein 9, Protein BOG, RBBP-10, RBBP-9

4 Images

Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

This data was developed using ab157202, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling BOG (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

This data was developed using ab157202, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BOG using ab157202 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

This data was developed using ab157202, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of MCF7 cells, labeling BOG using ab157202 at a 1/250 dilution.

Unknown

Western blot - Anti-BOG antibody [EPR9905] - BSA and Azide free (AB249307)

This data was developed using ab157202, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BOG antibody [EPR9905] (<a href='/products/primary-antibodies/bog-antibody-epr9905-ab157202'>ab157202</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 21 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-BOG antibody [EPR9905]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9905

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249307 is the carrier-free version of ab157202.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine hydrolase (Probable) (PubMed : 32196348). Catalyzes the hydrolytic activation of amino acid ester of the antiviral prodrug valacyclovir to its corresponding active drug, acyclovir (PubMed : 32196348). May negatively regulate basal or autocrine TGF-beta signaling by suppressing SMAD2-SMAD3 phosphorylation (PubMed : 20080647). May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta through interaction with RB1 and the subsequent displacement of E2F1 (PubMed : 9697699).

See full target information RBBP9

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-BOG抗体[EPR9905] - BSA and Azide free