重组Anti-BOB1抗体[EP3300] (ab76010)
![Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.cn/ps/products/76/ab76010/Images/ab76010-275024-anti-bob1-antibody-ep3300-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (ab76010)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3300] to BOB1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BOB1抗体[EP3300]
参阅全部 BOB1 一抗 -
描述
兔单克隆抗体[EP3300] to BOB1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human BOB1 aa 200-300. The exact sequence is proprietary.
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阳性对照
- Ramos cell lysate. Human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数（KD）
KD = 1.14 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3300 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76010于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| WB |
1/5000 - 1/30000. Predicted molecular weight: 28 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/5000 - 1/30000. Predicted molecular weight: 28 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
组织特异性
B-cell specific. -
疾病相关
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
序列相似性
Belongs to the POU2AF1 family. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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别名
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (ab76010)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling BOB1 with unpurified ab76010 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.cn/ps/products/76/ab76010/Images/ab76010-91290-anti-bob1-antibody-ep3300-western-blot.jpg)
Western blot - Anti-BOB1 antibody [EP3300] (ab76010)Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution + Ramos cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.cn/ps/products/76/ab76010/Images/ab76010-91289-anti-bob1-antibody-ep3300-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (ab76010)ab76010, at 1/100 dilution, staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.cn/ps/products/76/ab76010/Images/ab76010-91288-anti-bob1-antibody-ep3300.jpg)
OI-RD Scanning - Anti-BOB1 antibody [EP3300] (ab76010) -
![Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.cn/ps/products/76/ab76010/Images/ab76010-5-benefits-of-recombinant-antibodies.png)
Anti-BOB1 antibody [EP3300] (ab76010)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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