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Immunology Adaptive Immunity B Cells Non-CD

Anti-BOB1抗体(ab3426)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Western blot - Anti-BOB1 antibody (ab3426)

    Key features and details

    • Goat polyclonal to BOB1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-BOB1 antibody [EP3300] (ab76010)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-BOB1抗体
      参阅全部 BOB1 一抗
    • 描述

      山羊多克隆抗体to BOB1
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse
      预测可用于: Human
    • 免疫原

      Synthetic peptide: DAYALNHTLSVEGF, corresponding to C terminal amino acids 243-256 of Human BOB1.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • Mouse spleen lysate.
    • 常规说明

      Principal Names – POU2AF1; BOB1; OBF1; OCAB; OBF-1; POU domain, class 2, associating factor 1. Official Gene Symbol - POU2AF1. GenBank Accession Number – NP_006226. LocusLink ID - 5450 (human); 18985 (mouse). Gene Ontology terms - transcription cofactor activity; transcription co-activator activity; transcription activating factor; humoral immune response; transcription from Pol II promoter.

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
    • Concentration information loading...
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Immunology
      • Adaptive Immunity
      • B Cells
      • Non-CD
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • POU

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Mouse spleen tissue lysate - total protein (ab7937)
    • Recombinant Protein

      • Recombinant Human BOB1 protein (ab109849)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3426于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

    A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate.

    说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

    A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate.

    靶标

    • 功能

      Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
    • 组织特异性

      B-cell specific.
    • 疾病相关

      Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
    • 序列相似性

      Belongs to the POU2AF1 family.
    • 翻译后修饰

      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession Q16633 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5450 Human
      • Entrez Gene: 18985 Mouse
      • Omim: 601206 Human
      • SwissProt: Q16633 Human
      • SwissProt: Q64693 Mouse
      • Unigene: 654525 Human
      • Unigene: 897 Mouse
      • 别名

        • B cell Oct binding protein 1 antibody
        • B cell specific coactivator OBF 1 antibody
        • B cell specific coactivator OBF1 antibody
        • B-cell-specific coactivator OBF-1 antibody
        • BOB 1 antibody
        • BOB-1 antibody
        • OBF 1 antibody
        • OBF1 antibody
        • OBF1_HUMAN antibody
        • OCA B antibody
        • OCA-B antibody
        • OCAB antibody
        • OCT binding factor 1 antibody
        • OCT-binding factor 1 antibody
        • POU class 2 associating factor 1 antibody
        • POU domain class 2 associating factor 1 antibody
        • POU domain class 2-associating factor 1 antibody
        • Pou2af1 antibody
        see all

      图片

      • Western blot - Anti-BOB1 antibody (ab3426)
        Western blot - Anti-BOB1 antibody (ab3426)
        ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL). ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL).

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab3426?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3426 被引用在 2 文献中.

      • Russo MV  et al. Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury. Nat Immunol 19:442-452 (2018). PubMed: 29662169
      • Joza S  et al. Loss of semaphorin-neuropilin-1 signaling causes dysmorphic vascularization reminiscent of alveolar capillary dysplasia. Am J Pathol 181:2003-17 (2012). PubMed: 23063659

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      Please kindly advise whether the antibody ab3426 anti BOB 1 can be applied on paraffin sections.

      Read More

      Abcam community

      Verified customer

      Asked on May 27 2005

      Answer

      Thank you for your email. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please let us know.

      Read More

      Abcam Scientific Support

      回复于 May 27 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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