重组Anti-BNIP3L/NIX抗体[EPR4033] (ab109414)
![Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)](https://www.abcam.cn/ps/products/109/ab109414/Images/ab109414-122236-anti-bnip3l-antibody-epr4033-western-blot.jpg "Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4033] to BNIP3L/NIX
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BNIP3L/NIX抗体[EPR4033]
参阅全部 BNIP3L/NIX 一抗 -
描述
兔单克隆抗体[EPR4033] to BNIP3L/NIX -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Raji and K562 cell lysates; Human breast carcinoma tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4033 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109414于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3. -
序列相似性
Belongs to the NIP3 family. -
翻译后修饰
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. -
细胞定位
Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 665 Human
- Entrez Gene: 12177 Mouse
- Entrez Gene: 140923 Rat
- Omim: 605368 Human
- SwissProt: O60238 Human
- SwissProt: Q9Z2F7 Mouse
- Unigene: 131226 Human
- Unigene: 29820 Mouse
see all -
别名
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
see all
图片
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Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)All lanes : Anti-BNIP3L/NIX antibody [EPR4033] (ab109414) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)](https://www.abcam.cn/ps/products/109/ab109414/Images/ab109414-122235-anti-bnip3l-antibody-epr4033-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)](https://www.abcam.cn/ps/products/109/ab109414/Images/ab109414-3-benefits-of-recombinant-antibodies.png)
Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab109414 被引用在 12 文献中.
- Yu J et al. Effects and potential mechanism of Ca2+/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle. Int J Mol Med 50:N/A (2022). PubMed: 35929517
- Xie X et al. Cardiac-specific CGI-58 deficiency activates the ER stress pathway to promote heart failure in mice. Cell Death Dis 12:1003 (2021). PubMed: 34702801
- Chen M et al. Baicalein Mediates Mitochondrial Autophagy via miR-30b and the NIX/BNIP3 Signaling Pathway in Parkinson's Disease. Biochem Res Int 2021:2319412 (2021). PubMed: 34457363
- Simpson CL et al. NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell Rep 34:108689 (2021). PubMed: 33535046
- Dowdell AS et al. The HIF target ATG9A is essential for epithelial barrier function and tight junction biogenesis. Mol Biol Cell 31:2249-2258 (2020). PubMed: 32726170