Anti-BNIP3L/NIX抗体(ab8399)
Key features and details
- Rabbit polyclonal to BNIP3L/NIX
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BNIP3L/NIX抗体
参阅全部 BNIP3L/NIX 一抗 -
描述
兔多克隆抗体to BNIP3L/NIX -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
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阳性对照
- K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L/NIX (4).
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常规说明
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L/NIX, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L/NIX induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L/NIX interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L/NIX is localized in mitochondria. The messenger RNA of Bnip3L/NIX is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L/NIX and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
Primary antibody说明
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins. -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa. To reduce possible background load less sample and or use more diluted antibody.
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| ICC/IF | (1) |
Use at an assay dependent concentration. See Abreview.
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| IHC-P |
Use a concentration of 2 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa. To reduce possible background load less sample and or use more diluted antibody. |
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ICC/IF
Use at an assay dependent concentration. See Abreview. |
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IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3. -
序列相似性
Belongs to the NIP3 family. -
翻译后修饰
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. -
细胞定位
Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 665 Human
- Entrez Gene: 12177 Mouse
- Entrez Gene: 140923 Rat
- Omim: 605368 Human
- SwissProt: O60238 Human
- SwissProt: Q9Z2F7 Mouse
- Unigene: 131226 Human
- Unigene: 29820 Mouse
see all -
别名
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
see all
图片
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Western blot - Anti-BNIP3L/NIX antibody (ab8399)All lanes : Anti-BNIP3L/NIX antibody (ab8399) at 1 µg/ml
Lane 1 : K562 whole cell lysate with absence of immunogenic peptide
Lane 2 : K562 whole cell lysate with presence of immunogenic peptideThe approximate 40 kDa top band represents monomer of Bnip3L/NIX.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody (ab8399)ab8399 at 2µg/ml staining BNIP3L/NIX in human kidney tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody (ab8399)Immunofluorescence of Bnip3L/NIX in Human Kidney cells using ab8399 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab8399 被引用在 28 文献中.
- Shen Y et al. Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy. Clin Transl Med 12:e719 (2022). PubMed: 35092699
- Abdrakhmanov A et al. Receptor-Mediated Mitophagy Rescues Cancer Cells under Hypoxic Conditions. Cancers (Basel) 13:N/A (2021). PubMed: 34439183
- Zhu N et al. Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1a/BNIP3 Pathway. Front Pharmacol 11:367 (2020). PubMed: 32292345
- Yu X et al. Hyperoxia exposure arrests alveolarization in neonatal rats via PTEN-induced putative kinase 1-Parkin and Nip3-like protein X-mediated mitophagy disorders. Int J Mol Med 46:2126-2136 (2020). PubMed: 33125104
- Hu Q et al. MicroRNA-137 exerts protective effects on hypoxia-induced cell injury by inhibiting autophagy/mitophagy and maintaining mitochondrial function in breast cancer stem-like cells. Oncol Rep 44:1627-1637 (2020). PubMed: 32945512