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AB313835

重组Anti-BNIP3L/NIX抗体[HL1203] - BSA and Azide free

Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free

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Rabbit Recombinant Monoclonal BNIP3L/NIX antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BNIP3L conjugated to Keyhole Limpet Haemocyanin.

查看别名

BNIP3A, BNIP3H, NIX, BNIP3L, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)

Paraffin-embedded mouse kidney tissue stained for BNIP3L protein at mitochondria with ab313835 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)
  • WB

Supplier Data

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835) at 1/1000 dilution

Lane 1:

Untreated (–) HeLa whole cell extracts at 30 µg

Lane 2:

Treated (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)
  • WB

Supplier Data

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (AB313835)

All lanes:

Western blot - Anti-BNIP3L/NIX antibody [HL1203] - BSA and Azide free (ab313835) at 1/1000 dilution

All lanes:

Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1203

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Rat, Mouse

应用

IHC-P, ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human BNIP3L conjugated to Keyhole Limpet Haemocyanin.

O60238

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP : in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.
See full target information BNIP3L
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