重组Anti-BMX抗体[Y396] (ab32153)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y396] to BMX
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BMX抗体[Y396]
参阅全部 BMX 一抗 -
描述
兔单克隆抗体[Y396] to BMX -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human BMX aa 50-150. The exact sequence is proprietary.
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阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y396 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32153于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 78 kDa).
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说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 78 kDa). |
靶标
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功能
Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells. -
组织特异性
Preferentially expressed in epithelial and endothelial cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
结构域
SH2 domain mediates interaction with RUFY1. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 660 Human
- Entrez Gene: 12169 Mouse
- Entrez Gene: 367786 Rat
- Omim: 300101 Human
- SwissProt: P51813 Human
- SwissProt: P97504 Mouse
- Unigene: 495731 Human
- Unigene: 504 Mouse
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别名
- Bmx antibody
- BMX non receptor tyrosine kinase antibody
- BMX_HUMAN antibody
see all
图片
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Anti-BMX antibody [Y396] (ab32153) at 1/500 dilution + HeLa cell lysate
Predicted band size: 78 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab32153 被引用在 3 文献中.
- Peng J et al. EPHA3 regulates the multidrug resistance of small cell lung cancer via the PI3K/BMX/STAT3 signaling pathway. Tumour Biol 37:11959-11971 (2016). PubMed: 27101199
- Xiong Y et al. Endotoxin Tolerance Inhibits Lyn and c-Src Phosphorylation and Association with Toll-Like Receptor 4 but Increases Expression and Activity of Protein Phosphatases. J Innate Immun 8:171-84 (2016). PubMed: 26457672
- Fiallos E et al. Human cytomegalovirus gene expression in long-term infected glioma stem cells. PLoS One 9:e116178 (2014). PubMed: 25549333