Anti-BMPR1B抗体[MM0055-3E12] (ab78417)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR1B antibody [MM0055-3E12] (ab78417)](https://www.abcam.cn/ps/products/78/ab78417/Images/ab78417-94221-anti-bmpr1b-antibody-mm0055-3e12-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR1B antibody [MM0055-3E12] (ab78417)")
Key features and details
- Mouse monoclonal [MM0055-3E12] to BMPR1B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-BMPR1B抗体[MM0055-3E12]
参阅全部 BMPR1B 一抗 -
描述
小鼠单克隆抗体[MM0055-3E12] to BMPR1B -
宿主
Mouse -
特异性
ab78417 detects BMPR1B. No cross reactivity was found to BMPR1A (ALK3). -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant human BMPR1B extracellular domain
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS). -
克隆
单克隆 -
克隆编号
MM0055-3E12 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78417于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
|
| IHC-P |
1/50 - 1/200.
|
| 说明 |
|---|
|
WB
1/100 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
|
IHC-P
1/50 - 1/200. |
靶标
-
功能
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. -
疾病相关
Defects in BMPR1B are the cause of acromesomelic chondrodysplasia with genital anomalies (AMDGA) [MIM:609441]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers).
Defects in BMPR1B are a cause of brachydactyly type A2 (BDA2) [MIM:112600]. Brachydactylies (BDs) are a group of inherited malformations characterized by shortening of the digits due to abnormal development of the phalanges and/or the metacarpals. They have been classified on an anatomic and genetic basis into five groups, A to E, including three subgroups (A1 to A3) that usually manifest as autosomal dominant traits. BDA2 was described first in a large Norwegian kindred. BDA2 is caused by mutations in BMPR1B gene and studies demonstrate that these mutations function as dominant negatives in vitro and in vivo. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 658 Human
- Omim: 603248 Human
- SwissProt: O00238 Human
- Unigene: 598475 Human
-
别名
- Activin receptor like kinase 6 antibody
- Acvrlk6 antibody
- ALK 6 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMPR1B antibody [MM0055-3E12] (ab78417)
ab78417 at 1/200 dilution staining BMPR1B in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
-
![Western blot - Anti-BMPR1B antibody [MM0055-3E12] (ab78417)](https://www.abcam.cn/ps/products/78/ab78417/Images/ab78417-94220-anti-bmpr1b-antibody-mm0055-3e12-western-blot.jpg)
Western blot - Anti-BMPR1B antibody [MM0055-3E12] (ab78417)All lanes : Anti-BMPR1B antibody [MM0055-3E12] (ab78417) at 1 µg/ml
Lane 1 : Human bone tumor tissue lysate - total protein (ab29359)
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 30 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The band observed at 53 kDa could potentially be a cleaved form of BMPR1B due to presence of a 13 amino acid signal peptide.
实验方案
数据表及文件
-
Datasheet download
文献 (7)
ab78417 被引用在 7 文献中.
- Tengroth L et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). PubMed: 29367682
- Wang M et al. Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis. Cell Stem Cell 23:599-614.e4 (2018). PubMed: 30174296
- Wang T et al. A Selective Cell Population from Dermis Strengthens Bone Regeneration. Stem Cells Transl Med 6:306-315 (2017). PubMed: 28170187
- Chapellier M et al. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response. Stem Cell Reports 4:239-54 (2015). IHC-P ; Human . PubMed: 25601208
- Osório C et al. Growth differentiation factor 5 is a key physiological regulator of dendrite growth during development. Development 140:4751-62 (2013). WB, ICC/IF ; Mouse . PubMed: 24173804
- Schmal H et al. Immunohistological localization of BMP-2, BMP-7, and their receptors in knee joints with focal cartilage lesions. ScientificWorldJournal 2012:467892 (2012). PubMed: 22272175
- Sinclair DC et al. Leiomyoma Simultaneously Impair Endometrial BMP-2-Mediated Decidualization and Anticoagulant Expression through Secretion of TGF-{beta}3. J Clin Endocrinol Metab 96:412-21 (2011). IHC-P ; Human . PubMed: 21084396
