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重组RabMAb

重组Anti-BMP4抗体[EPR6211] (ab124715)

Submit a review Submit a question References (26)
Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
  • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Anti-BMP4 antibody [EPR6211] (ab124715)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6211] to BMP4
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Carrier Free HRP

概述

  • 产品名称

    Anti-BMP4抗体[EPR6211]
    参阅全部 BMP4 一抗

  • 描述

    兔单克隆抗体[EPR6211] to BMP4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human BMP4 aa 350 to the C-terminus.

  • 阳性对照

    • WB: Human fetal liver, A549, HeLa, A431, and Human heart lysates IP: A549 whole cell lysate. IHC-P: Human breast cancer, and colon Tissue Flow Cyt (intra): HeLa cells ICC/IF: A549 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124715于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
IP
1/10 - 1/100.
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols.
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100. 
说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
IP
1/10 - 1/100.
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols.
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100. 

靶标

  • 功能

    Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

  • 组织特异性

    Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

  • 疾病相关

    Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.

  • 序列相似性

    Belongs to the TGF-beta family.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • zgc:100779 antibody
      • BMP 2B antibody
      • BMP 4 antibody
      • BMP-2B antibody
      • BMP-4 antibody
      • BMP2B antibody
      • BMP2B1 antibody
      • BMP4 antibody
      • BMP4_HUMAN antibody
      • Bone morphogenetic protein 2B antibody
      • Bone morphogenetic protein 4 antibody
      • DVR4 antibody
      • MCOPS6 antibody
      • MGC100779 antibody
      • OFC11 antibody
      • zbmp-4 antibody
      • ZYME antibody
      see all

    图片

    • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Anti-BMP4 antibody [EPR6211] (ab124715) at 0.05 µg/ml (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 47 kDa



      Blocking and diluting buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling BMP4 with Purified ab124715 at 1:400 dilution (2.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)

      Unpurified ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (ab124715)

      ab124715 (purified) at 1:60 dilution (2µg) immunoprecipitating BMP4 in A549 whole cell lysate.
      Lane 1 (input): A549 (Human lung carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab124715 & A549 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124715 in A549 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] (ab124715)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BMP4 with purified ab124715 at 1/120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] (ab124715)

      Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) cells labelling BMP4 with purified ab124715 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      All lanes : Anti-BMP4 antibody [EPR6211] (ab124715) at 1/10000 dilution (Unpurified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : A549 lysate
      Lane 3 : HeLa lysate
      Lane 4 : A431 lysate
      Lane 5 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] (ab124715)
      OI-RD Scanning - Anti-BMP4 antibody [EPR6211] (ab124715)

      Produced using unpurified ab124715

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    • Anti-BMP4 antibody [EPR6211] (ab124715)
      Anti-BMP4 antibody [EPR6211] (ab124715)

    文献 (26)

    发表研究结果有使用 ab124715？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124715 被引用在 26 文献中.

    • Oguić M  et al. Osteogenic Potential of Autologous Dentin Graft Compared with Bovine Xenograft Mixed with Autologous Bone in the Esthetic Zone: Radiographic, Histologic and Immunohistochemical Evaluation. Int J Mol Sci 24:N/A (2023). PubMed: 37047413
    • Ma B  et al. High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist. BMC Cancer 23:594 (2023). PubMed: 37370018
    • Camdzic N  et al. The Impact of BMP-4 Tissue Expression on Progression and Survival in Breast Cancer. Med Arch 77:207-212 (2023). PubMed: 37700913
    • Wang J  et al. KLF4 Alleviates Hypertrophic Scar Fibrosis by Directly Activating BMP4 Transcription. Int J Biol Sci 18:3324-3336 (2022). PubMed: 35637963
    • Li S  et al. Selective targeting BMP2 and 4 in SMAD4 negative esophageal adenocarcinoma inhibits tumor growth and aggressiveness in preclinical models. Cell Oncol (Dordr) 45:639-658 (2022). PubMed: 35902550
    View all Publications for this product

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