Anti-BMP4抗体(ab39973)
Key features and details
- Rabbit polyclonal to BMP4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BMP4抗体
参阅全部 BMP4 一抗 -
描述
兔多克隆抗体to BMP4 -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab39973 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab124715.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Pig -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BMP4.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39973于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. -
组织特异性
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. -
疾病相关
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 652 Human
- Entrez Gene: 12159 Mouse
- Entrez Gene: 25296 Rat
- Omim: 112262 Human
- SwissProt: P12644 Human
- SwissProt: P21275 Mouse
- SwissProt: Q06826 Rat
- Unigene: 68879 Human
see all -
别名
- zgc:100779 antibody
- BMP 2B antibody
- BMP 4 antibody
see all
图片
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All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab39973)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of ab39973 staining primary Rat hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human brain tissue lysate - total protein (ab29466) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 7 : Brain (Mouse) Mouse Tissue Lysate with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 8 : Human liver tissue lysate - total protein (ab29889) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 9 : Human heart tissue lysate - total protein (ab29431) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 10 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-BMP4 antibody (ab39973)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomAll lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution
All lanes : mouse brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 1 minute
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (98)
ab39973 被引用在 98 文献中.
- Shen W et al. Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury. Dis Model Mech 16:N/A (2023). PubMed: 36518009
- Woodward E et al. Characterisation of the testicular transcriptome in stallions with age-related testicular degeneration. Equine Vet J 55:239-252 (2023). PubMed: 35569039
- Su X et al. Activin A rescues preterm brain injury through a novel Noggin/BMP4/Id2 signaling pathway. Int J Mol Med 51:N/A (2023). PubMed: 36524372
- Gao LT et al. Hypermethylation of the Bmp4 promoter dampens binding of HIF-1α and impairs its cardiac protective effects from oxidative stress in prenatally GC-exposed offspring. Cell Mol Life Sci 80:58 (2023). PubMed: 36746787
- Wang C et al. Smad4-mediated angiogenesis facilitates the beiging of white adipose tissue in mice. iScience 26:106272 (2023). PubMed: 36915676