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Cardiovascular Angiogenesis Growth Factors TGF

Anti-BMP4抗体(ab39973)

  • Datasheet
  • SDS
Reviews (14)Q&A (6)References (87)

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Western blot - Anti-BMP4 antibody (ab39973)
  • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab39973)
  • Western blot - Anti-BMP4 antibody (ab39973)
  • Western blot - Anti-BMP4 antibody (ab39973)

Key features and details

  • Rabbit polyclonal to BMP4
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-BMP4 antibody [EPR6211] (ab124715)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-BMP4抗体
    参阅全部 BMP4 一抗
  • 描述

    兔多克隆抗体to BMP4
  • 宿主

    Rabbit
  • 特异性

    Replenishment batches of our polyclonal antibody, ab39973 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab124715.

  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BMP4.

    参阅Abcam的专有抗源政策 (Peptide available as ab40140.)
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Secreted
    • Cancer
    • Growth factors
    • TGF
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Gut specification
    • Developmental Biology
    • Organogenesis
    • Nervous system development
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human BMP4 protein (ab87063)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab39973于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF (1)
Use a concentration of 1 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
  • 组织特异性

    Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
  • 疾病相关

    Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
  • 序列相似性

    Belongs to the TGF-beta family.
  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P12644 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 652 Human
    • Entrez Gene: 12159 Mouse
    • Entrez Gene: 25296 Rat
    • Omim: 112262 Human
    • SwissProt: P12644 Human
    • SwissProt: P21275 Mouse
    • SwissProt: Q06826 Rat
    • Unigene: 68879 Human
    • Unigene: 6813 Mouse
    • Unigene: 10318 Rat
    see all
  • 别名

    • zgc:100779 antibody
    • BMP 2B antibody
    • BMP 4 antibody
    • BMP-2B antibody
    • BMP-4 antibody
    • BMP2B antibody
    • BMP2B1 antibody
    • BMP4 antibody
    • BMP4_HUMAN antibody
    • Bone morphogenetic protein 2B antibody
    • Bone morphogenetic protein 4 antibody
    • DVR4 antibody
    • MCOPS6 antibody
    • MGC100779 antibody
    • OFC11 antibody
    • zbmp-4 antibody
    • ZYME antibody
    see all

图片

  • Western blot - Anti-BMP4 antibody (ab39973)
    Western blot - Anti-BMP4 antibody (ab39973)
    All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab39973)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab39973)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    ICC/IF image of ab39973 staining primary Rat hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-BMP4 antibody (ab39973)
    Western blot - Anti-BMP4 antibody (ab39973)
    All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 6 : Human brain tissue lysate - total protein (ab29466) with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 7 : Brain (Mouse) Mouse Tissue Lysate with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 8 : Human liver tissue lysate - total protein (ab29889) with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 9 : Human heart tissue lysate - total protein (ab29431) with Human BMP4 peptide (ab40140) at 1 µg/ml
    Lane 10 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human BMP4 peptide (ab40140) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 47 kDa



    ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Western blot - Anti-BMP4 antibody (ab39973)
    Western blot - Anti-BMP4 antibody (ab39973)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
    All lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution

    All lanes : mouse brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Dako Cytomation P0260
    Conjugation: HRP at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa


    Exposure time: 1 minute

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (87)

发表研究结果有使用 ab39973?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab39973 被引用在 87 文献中.

  • Shen W  et al. Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury. Dis Model Mech 16:N/A (2023). PubMed: 36518009
  • Skauli N  et al. Canonical Bone Morphogenetic Protein Signaling Regulates Expression of Aquaporin-4 and Its Anchoring Complex in Mouse Astrocytes. Front Cell Neurosci 16:878154 (2022). PubMed: 35518645
  • Li T  et al. BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging. Biomedicines 10:N/A (2022). PubMed: 35625884
  • Wu T  et al. Role of Bone Morphogenetic Protein 4 in the Inflammation of the Myocardium and Vascular Tissue of Obese Mice. J Cardiovasc Pharmacol 79:791-798 (2022). PubMed: 35666994
  • Zhang J  et al. Augmented BMP4 signal impairs tongue myogenesis. J Mol Histol 52:651-659 (2021). PubMed: 34076834
View all Publications for this product

客户评价及客户问答

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1-10 of 20 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP4 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA high pH
Permeabilization
Yes - Tween 20
Specification
Brain
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 31 2019

Immunohistochemistry (Frozen sections) abreview for Anti-BMP4 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone)
Permeabilization
No
Specification
Bone
Blocking step
Vector Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 14 2019

Immunohistochemistry (Frozen sections) abreview for Anti-BMP4 antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (bone marrow)
Permeabilization
Yes - Triton
Specification
bone marrow
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

提交于 Sep 16 2019

Immunohistochemistry (Frozen sections) abreview for Anti-BMP4 antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 28 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP4 antibody

Inconclusive
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (NF stage embryos)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - PBT (0.1% Triton in PBS)
Specification
NF stage embryos
Blocking step
(agent) for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 07 2015

Western blot abreview for Anti-BMP4 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Cell lysate - whole cell (Gastrocnemius)
Specification
Gastrocnemius
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Francesco Elia Marino

Verified customer

提交于 Apr 11 2014

Question

This antibody is not working in WB. Can I please receive a replacement?

Read More

Abcam community

Verified customer

Asked on May 28 2012

Answer

Thank you for contacting Abcam.


I have requested that a vial of ab124715 be shipped out immediately in Cedarlane's next order. I will confirm this shortly.


I apologize for any inconvenience this has caused. Please do not hesitate to contact me if you have any additonal questions.

Read More

Abcam Scientific Support

回复于 May 28 2012

Question

ab58803 gave no bands in WB using positive control ab3871
ab39973 gave no bands in WB using positive control ab29466

Read More

Abcam community

Verified customer

Asked on Feb 10 2012

Answer

Thank you for contacting us.

As we discussed on the phone, you may be able to improve your WB results by switching from milk to BSA and by using a higher concentration of primary antibody (1:500 for example). If you are unable to improve your results, please let me know and I will be happy to offer you either replacements with alternative antibodies or credits on your original purchase. Please let me know how you would like to proceed and I will be happy to assist you further.

Read More

Abcam Scientific Support

回复于 Feb 10 2012

Question

I would like to test ab39973 in IHC-Fronpig tissue. Could you please send information about the 100% Abreview discount?

Read More

Abcam community

Verified customer

Asked on Jan 17 2012

Answer

DISCOUNT CODE:


Expiration date:

I am very pleased to hear you would like to accept our offer and test ab39973 inIHC-Fr on pig. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-Fr onpigand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

回复于 Jan 17 2012

Question

Dear Abcam. What is the homology of the imunogen and this ovine sequence, Accession: ABW37747.1 ? Kind regards

Read More

Abcam community

Verified customer

Asked on Jan 13 2012

Answer

Thank you very much for your interest in ab39973. I can confirm that this antibody has not yet been tested with sheep samples The immunogen shows 84% homology with the sequence you are interested in. We usually expect a cross-reaction for an antibody from 85% homology and up. I am aware that this homology is only boarder line, but would still like to offer a discount off a future purchase if you buy ab39973 now, test it in Ovis aries (sheep) and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab39973 in sheep. I will then send a discount code. This code must be issued before purchasing ab39973 so please wait for my reply before ordering. 2. Purchase ab39973 either by phone, fax, or online (www.abcam.com). 3. Test it in sheep. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab39973 turns out to be unsuitable for sheep, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

回复于 Jan 13 2012

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