Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-BMP1/PCP抗体(ab38953)

Reviews (3)Q&A (3)References (5)

Key features and details

  • Rabbit polyclonal to BMP1/PCP
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-BMP1/PCP抗体
    参阅全部 BMP1/PCP 一抗

  • 描述

    兔多克隆抗体to BMP1/PCP

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human BMP1/PCP (C terminal).
    (Peptide available as ab41291)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab38953于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 111 kDa.

A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 111 kDa.

A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

靶标

  • 功能

    Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Responsible for the proteolytic activation of lysyl oxidase LOX.

  • 组织特异性

    Ubiquitous.

  • 疾病相关

    Osteogenesis imperfecta 13

  • 序列相似性

    Belongs to the peptidase M12A family.
    Contains 5 CUB domains.
    Contains 2 EGF-like domains.

  • 翻译后修饰

    Proteolytically activated in the trans-Golgi network by furin-like/paired basic proprotein convertases, cleavage is not required for secretion.

  • 细胞定位

    Golgi apparatus, trans-Golgi network. Secreted, extracellular space, extracellular matrix. Co-localizes with POSTN in the Golgi.
  • Information by UniProt

  • 数据库链接

    • 别名

      • BMP 1 antibody
      • BMP-1 antibody
      • BMP1 antibody
      • BMP1_HUMAN antibody
      • Bone morphogenetic protein 1 antibody
      • Mammalian tolloid protein antibody
      • mTld antibody
      • OI13 antibody
      • PCOLC antibody
      • PCP antibody
      • PCP2 antibody
      • Procollagen C endopeptidase antibody
      • Procollagen C proteinase antibody
      • Procollagen C-proteinase antibody
      • TLD antibody
      • Tolloid, Drosophila, homolog of antibody
      see all

    文献 (5)

    发表研究结果有使用 ab38953？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab38953 被引用在 5 文献中.

    • Kazakevych J  et al. Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium. Genome Biol 21:64 (2020). PubMed: 32160911
    • Jameson SA  et al. Elastin homeostasis is altered with pelvic organ prolapse in cultures of vaginal cells from a lysyl oxidase-like 1 knockout mouse model. Physiol Rep 8:e14436 (2020). PubMed: 32533648
    • Voorhees AP  et al. Building a better infarct: Modulation of collagen cross-linking to increase infarct stiffness and reduce left ventricular dilation post-myocardial infarction. J Mol Cell Cardiol 85:229-39 (2015). PubMed: 26080361
    • Zamilpa R  et al. CC chemokine receptor 5 deletion impairs macrophage activation and induces adverse remodeling following myocardial infarction. Am J Physiol Heart Circ Physiol 300:H1418-26 (2011). WB ; Mouse . PubMed: 21297029
    • Ahn Y  et al. Mining of serum glycoproteins by an indirect approach using cell line secretome. Mol Cells 29:123-30 (2010). WB ; Human . PubMed: 20016938

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-BMP1/PCP antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (concentrated secreted media of HeLa cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    1e+006 cells
    Specification
    concentrated secreted media of HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 03 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BMP1/PCP antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% Triron X100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 27 2017

    Western blot abreview for Anti-BMP1/PCP antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Sebastian Brings

    Verified customer

    提交于 Oct 21 2011

    Question

    Read More
    Answer

    Thank you for your email. The Abreview discount code is now valid as you the Abreview has been received and published. Please submit your order with the discount code for it to be applied to your next order. Thank you for your feedback about our products.

    Read More

    Question

    Dear Ladies and Gentleman, I would like to know whether the antibody for BMP1 is also suitable for the use in rats. Do you have any experience with that? In case you don't could you please send me the peptide sequence that the ab was raised against so that I can run a sequence comparison. Cheers,

    Read More
    Answer

    Thank you very much for your interest in ab38953. To our knowledge, ab38953 has not been tested in rat. Unfortunately I can not provide a sequence alignment neither, as the immunogen sequence is proprietary information of the laboratory producing this antibody and I do not have access. I am very sorry. I have however aligned the whole sequence of mouse to the rat sequence (as we know this antibody reacts with mouse) and the sequences seem to be very conserved. http://www.uniprot.org/align/2011082560LBQDGUB8 So I would suggest that crossreactivity is likely. However, this needs to be assessed experimentally. If you would like to test this, I can offer a discount off a future purchase if you buy ab38953 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab38953 in rat. I will then send a discount code. This code must be issued before purchasing ab38953 so please wait for my reply before ordering. 2. Purchase ab38953 either by phone, fax, or online (www.abcam.com). 3. Test it in rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab38953 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    What was the lysate used to test this antibody in Western blots?

    Read More
    Answer

    Thank you for your enquiry. The lysates used to test this antibody for Western blotting were from RD cells and A2058 cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com