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AB247662

重组Anti-BMP15抗体[EPR5137] - BSA and Azide free

Anti-BMP15 antibody [EPR5137] - BSA and Azide free

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Rabbit Recombinant Monoclonal BMP15 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

查看别名

GDF9B, BMP15, Bone morphogenetic protein 15, BMP-15, Growth/differentiation factor 9B, GDF-9B

3 Images
Flow Cytometry (Intracellular) - Anti-BMP15 antibody [EPR5137] - BSA and Azide free (AB247662)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BMP15 antibody [EPR5137] - BSA and Azide free (AB247662)

This data was developed using ab108413, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of BMP15 in permeabilized Hela cells using ab108413 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-BMP15 antibody [EPR5137] - BSA and Azide free (AB247662)
  • WB

Unknown

Western blot - Anti-BMP15 antibody [EPR5137] - BSA and Azide free (AB247662)

This data was developed using ab108413, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BMP15 antibody [EPR5137] (<a href='/products/primary-antibodies/bmp15-antibody-epr5137-ab108413'>ab108413</a>) at 1/1000 dilution

Lane 1:

Ovary cancer tissue lysate at 10 µg

Lane 2:

Uterus tissue lysate at 10 µg

Lane 3:

HL-60 cell lysate at 10 µg

Lane 4:

NIH:OVCAR-3 cell lysate at 10 µg

Lane 5:

Human testis tissue lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Predicted band size: 45 kDa

false

OI-RD Scanning - Anti-BMP15 antibody [EPR5137] - BSA and Azide free (AB247662)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-BMP15 antibody [EPR5137] - BSA and Azide free (AB247662)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5137

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

ab247662 is the carrier-free version of ab108413.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.
See full target information BMP15
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Abcam Product Promise

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