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Anti-BMAL1抗体(ab3350)

  • Datasheet
  • SDS
Reviews (5)Q&A (3)References (63)

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Immunocytochemistry - Anti-BMAL1 antibody (ab3350)
  • Western blot - Anti-BMAL1 antibody (ab3350)
  • Western blot - Anti-BMAL1 antibody (ab3350)

Key features and details

  • Rabbit polyclonal to BMAL1
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-BMAL1 antibody [EPR23696-22] (ab230822)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-BMAL1抗体
    参阅全部 BMAL1 一抗
  • 描述

    兔多克隆抗体to BMAL1
  • 宿主

    Rabbit
  • 特异性

    Detects BMAL 1 / aryl hydrocarbon nuclear translocator 3 (ARNT 3) from hamster and mouse tissues as well as recombinant human BMAL 1.

  • 经测试应用

    适用于: WB, ICCmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Horse, Chicken, Syrian hamster
  • 免疫原

    Synthetic peptide corresponding to Mouse BMAL1 aa 582-594.
    Sequence:

    DMIDNDQGSSSPS


    (Peptide available as ab4959)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: human U251 and U87-MG, mouse NIH-3T3 cell lines; human SH-SY5Y, U-87 MG, U-2 OS, HeLa, HEK293,and JURKAT cells, and rat brain tissue; ICC: SH-SY5Y cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Neuroscience
    • Neurology process
    • Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • BMAL1 peptide (ab4959)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human BMAL1 protein (ab131888)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3350于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/200. Predicted molecular weight: 69.4 kDa.Can be blocked with BMAL1 peptide (ab4959). This antibody detects a protein which corresponds to the product of a hamster BMAL 1 fusion construct overexpressed in E. coli (110 kDa band)as well as recombinant human BMAL1.
ICC
Use a concentration of 2 µg/ml.
说明
WB
1/200. Predicted molecular weight: 69.4 kDa.Can be blocked with BMAL1 peptide (ab4959). This antibody detects a protein which corresponds to the product of a hamster BMAL 1 fusion construct overexpressed in E. coli (110 kDa band)as well as recombinant human BMAL1.
ICC
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK
    NPAS2-ARNTL/BMAL1
    ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. ARNTL/BMAL1 positively regulates myogenesis and negatively regulates adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. Plays a role in normal pancreatic beta-cell function; regulates glucose-stimulated insulin secretion via the regulation of antioxidant genes NFE2L2/NRF2 and its targets SESN2, PRDX3, CCLC and CCLM. Negatively regulates the mTORC1 signaling pathway; regulates the expression of MTOR and DEPTOR. Controls diurnal oscillations of Ly6C inflammatory monocytes; rhythmic recruitment of the PRC2 complex imparts diurnal variation to chemokine expression that is necessary to sustain Ly6C monocyte rhythms. Regulates the expression of HSD3B2, STAR, PTGS2, CYP11A1, CYP19A1 and LHCGR in the ovary and also the genes involved in hair growth. Plays an important role in adult hippocampal neurogenesis by regulating the timely entry of neural stem/progenitor cells (NSPCs) into the cell cycle and the number of cell divisions that take place prior to cell-cycle exit. Regulates the circadian expression of CIART and KLF11. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1, VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1, PPARGC1A, PPARGC1B, SIRT1, GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1, ATF4, MTA1, KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, regulating the DNA accessibility of other transcription factors. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina.
  • 组织特异性

    Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
  • 序列相似性

    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻译后修饰

    Ubiquitinated, leading to its proteasomal degradation.
    O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.
    Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.
    Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry.
    Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.
  • 细胞定位

    Nucleus. Cytoplasm. Nucleus, PML body. Shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. The sumoylated form localizes in the PML body. Sequestered to the cytoplasm in the presence of ID2.
  • Target information above from: UniProt accession O00327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 374115 Chicken
    • Entrez Gene: 406 Human
    • Entrez Gene: 11865 Mouse
    • Entrez Gene: 29657 Rat
    • Omim: 602550 Human
    • SwissProt: O00327 Human
    • SwissProt: Q9WTL8 Mouse
    • SwissProt: Q9EPW1 Rat
    • Unigene: 65734 Human
    • Unigene: 33970 Mouse
    • Unigene: 440371 Mouse
    • Unigene: 14532 Rat
    see all
  • 别名

    • ARNT like protein 1 brain and muscle antibody
    • Arntl antibody
    • Aryl hydrocarbon receptor nuclear translocator like antibody
    • Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody
    • Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody
    • Basic helix loop helix PAS orphan MOP3 antibody
    • Basic helix loop helix PAS protein MOP3 antibody
    • Basic-helix-loop-helix-PAS protein MOP3 antibody
    • bHLH PAS protein JAP3 antibody
    • bHLH-PAS protein JAP3 antibody
    • bHLHe5 antibody
    • BMAL 1 antibody
    • BMAL1_HUMAN antibody
    • BMAL1c antibody
    • Brain and muscle ARNT like 1 antibody
    • Brain and muscle ARNT-like 1 antibody
    • CG8727 PA antibody
    • Class E basic helix-loop-helix protein 5 antibody
    • cycle antibody
    • JAP 3 antibody
    • JAP3 antibody
    • Member of PAS protein 3 antibody
    • Member of PAS superfamily 3 antibody
    • MGC47515 antibody
    • MOP 3 antibody
    • MOP3 antibody
    • PAS domain-containing protein 3 antibody
    • PASD 3 antibody
    • PASD3 antibody
    • TIC antibody
    see all

图片

  • Immunocytochemistry - Anti-BMAL1 antibody (ab3350)
    Immunocytochemistry - Anti-BMAL1 antibody (ab3350)

    Immunocytochemistry analysis of  4% paraformaldehyde-fixed SH-SY5Y cells permeabilised with 0.1% Triton™ X-100 (10 mins) and blocked with 1% BSA (1  hour).

    a) BMAL1 is stained with ab3350 at 2µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate in 1/2000 dilution (green). b) Nuclear staining with DAPI and SlowFade® Gold Antifade Mountant (blue). c) F-actin stainined with a 1/3000 dilution Alexa Fluor® 555 Rhodamine Phalloidin (red). d) merged image. e) control with no primary antibody.

  • Western blot - Anti-BMAL1 antibody (ab3350)
    Western blot - Anti-BMAL1 antibody (ab3350)
    All lanes : Anti-BMAL1 antibody (ab3350) at 1/500 dilution

    Lane 1 : U251 cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : NIH-3T3 cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 69.4 kDa
    Observed band size: 69 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-BMAL1 antibody (ab3350)
    Western blot - Anti-BMAL1 antibody (ab3350)
    Anti-BMAL1 antibody (ab3350) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 69.4 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?
    Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    The 75 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to BMAL1.

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (63)

发表研究结果有使用 ab3350?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3350 被引用在 63 文献中.

  • Rakshit K & Matveyenko AV Induction of Core Circadian Clock Transcription Factor Bmal1 Enhances ß-Cell Function and Protects Against Obesity-Induced Glucose Intolerance. Diabetes 70:143-154 (2021). PubMed: 33087455
  • Zhang SL  et al. A circadian clock regulates efflux by the blood-brain barrier in mice and human cells. Nat Commun 12:617 (2021). PubMed: 33504784
  • Zhuang X  et al. Circadian control of hepatitis B virus replication. Nat Commun 12:1658 (2021). PubMed: 33712578
  • Maury E  et al. Circadian clock dysfunction in human omental fat links obesity to metabolic inflammation. Nat Commun 12:2388 (2021). PubMed: 33888702
  • Bertile F  et al. Daytime Restricted Feeding Affects Day-Night Variations in Mouse Cerebellar Proteome. Front Mol Neurosci 14:613161 (2021). PubMed: 33912010
View all Publications for this product

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BMAL1 antibody - ChIP Grade

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (Hypothalamic Suprachiasmatic Nucleus)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Hypothalamic Suprachiasmatic Nucleus
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kevin Zhang

Verified customer

提交于 May 16 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-BMAL1 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hepatocytes)
Permeabilization
Yes - : 0.1% Triton X-100 in TBS for 5-10 minutes
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 11 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-BMAL1 antibody - ChIP Grade

Below Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cell (Hepatocyte)
Specification
Hepatocyte
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 20 2014

Western blot abreview for Anti-BMAL1 antibody - ChIP Grade

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 05 2013

Immunofluorescence abreview for Anti-BMAL1 antibody - ChIP Grade

Poor
Abreviews
Abreviews
Application
Immunofluorescence
Sample
Mouse Tissue sections (Brain Suprachiasmatic nuclei)
Specification
Brain Suprachiasmatic nuclei
Fixative
Paraformaldehyde
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 10 2005

Question

Many thanks for your quick answer.
I have talked things over with my boss, and she agreed to purchase the positive control, which will be really helpful.

However, I am not sure about which HeLa lysate to order.
On your website, I've found two different products, both named '
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate'. One has the code ab29545, and the other one ab150035. Both appear to be suitable for Western Blotting.
Can you advise me on which one of these lysates is best to use as a BMAL1 positive control?
After that, I can order it through our internal ordering system.

Many thanks in advance.

Best regards,

Read More

Abcam community

Verified customer

Asked on Dec 06 2012

Answer

Thank you for contacting us.

I could suggest buying ab150035.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

回复于 Dec 06 2012

Question

Product code: 3350
Lot number: GR49442-1
Inquiry: Dear sir/madam, Some time ago, I purchased the anti-BMAL1 antibody (ab3350) from your company. Initially, I was not able to see the predicted band using WB, but after some time of optimization, I seem to have succeeded in visualizing some (faint) bands a little lower than the 70 kD mark in murine heart tissue lysates. However, as of yet I have not seen the same band in the positive control suggested in the datasheet, i.e. HeLa whole cell lysate, which makes it difficult to be certain of the correct height of seen bands. Would it be possible for you to send me a positive control (protein or lysate), guaranteed to show a band, so I can ascertain myself of the correctness of these bands? If so, I would be very much obliged. For your information, I enclosed a picture of some blots, for which I used different conditions. Other conditions I have tried I will list below: * amount of protein loaded: 20 - 40 - 60 µg * blocking conditions: 20 min.@RT in 5% milk - 5% BSA * detection method: ECL (Amersham) * electrophoresis: 10% PAA gels (0.75 mm), ∼2.5 h at 120 V, samples boiled and loaded in sample buffer containing β-mercaptoethanol * transfer conditions: PVDF membranes, 1.5 h at 120 V in blotting buffer (2% methanol), cooled and stirred * primary antibody conditions: 1/500 - 1/1000 in 5% milk - 5% BSA, overnight@4°C * secondary antibody conditions: Swine anti-Rabbit 1/1000 in 5% milk - 5% BSA, 2 h@RT * wash steps: 3x15 min.@RT with TBS-Tween (after 1st AB and after 2nd AB) Many thanks in advance! Kind regards,

Read More

Abcam community

Verified customer

Asked on Nov 28 2012

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

This antibody has been tested with Hela cells and is guaranteed for working in WB including other cell line lysates. We can certainly provide the Hela cell lysates however these will be general lysates i.e. not the one which we used to test this antibody. Let me know if you are OK to purchase these.

I have further read the details you have kindly provided and have following further questions for better understanding of the problem;

- How the antibody was stored?
- When the antibody was ordered? Could you provide the purchase order number?
- I agree the antibody does seem detecting very faint band at 70kDa. Could you provide details which lysis buffer was used and how the tissue lysates were prepared.

This protein has 5 isoforms (http://www.uniprot.org/uniprot/Q9WTL8) the antibody will detect all the isoforms if they are expressed. Please check literature about the published isofiorms in murine heart tissue.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Abcam Scientific Support

回复于 Nov 28 2012

Question

Do you have any images of a western using this antibody and the predicted molecular weight? Thank you for your help!

Read More

Abcam community

Verified customer

Asked on Jun 15 2005

Answer

Thank you for your enquiry. We currently do not have an image of the western blot with ab3350, all the information, including images, we have for all our products is listed on the on-line datasheets for your convenience and we update these as soon as we get more data. Ab3350 was tested on hamster BMAL1/aryl hydrocarbon nuclear translocator 3 (ARNT3)fusion construct overexpressed in E. coli (a 110 kDa protein) as well as recombinant human BMAL1. The predicted MW of BMAL1 is 69.4 kDa. Please do not hesitate to contact us again if you need further assistance,

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回复于 Jun 15 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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