为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.

Anti-Blooms Syndrome蛋白Blm抗体(ab2179)

  • Datasheet
  • SDS
Reviews (12)Q&A (5)References (38)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
  • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
  • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

Key features and details

  • Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

你可能会感兴趣

偶联
Antibody Concentration And Clean-Up Kit – Gold And Magnetic Nanoparticles (ab204911)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一抗
Product image
Anti-Ube2N / Ubc13 antibody (ab25885)

查看更多相关产品

概述

  • 产品名称

    Anti-Blooms Syndrome蛋白Blm抗体
    参阅全部 Blooms Syndrome Protein Blm 一抗
  • 描述

    兔多克隆抗体to Blooms Syndrome蛋白Blm
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IPmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK-293T, K-562, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: Blooms Syndrome Protein Blm in HEK-293T whole cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2179于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (6)
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
IP (1)
Use at 2-10 µg/mg of lysate.
说明
WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
IP
Use at 2-10 µg/mg of lysate.

靶标

  • 功能

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • 疾病相关

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • 序列相似性

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻译后修饰

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P54132 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 641 Human
    • Entrez Gene: 12144 Mouse
    • Omim: 604610 Human
    • SwissProt: P54132 Human
    • SwissProt: O88700 Mouse
    • Unigene: 725208 Human
    • 别名

      • Blm antibody
      • BLM_HUMAN antibody
      • Bloom syndrome antibody
      • Bloom syndrome protein antibody
      • Bloom syndrome RecQ helicase like antibody
      • BS antibody
      • DNA helicase antibody
      • DNA helicase RecQ like type 2 antibody
      • MGC126616 antibody
      • MGC131618 antibody
      • MGC131620 antibody
      • RECQ 2 antibody
      • RECQ like antibody
      • RecQ like type 2 antibody
      • RecQ protein like 3 antibody
      • RecQ protein-like 3 antibody
      • RecQ-like type 2 antibody
      • RecQ2 antibody
      • RECQL 2 antibody
      • RECQL 3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • type 2 antibody
      see all

    图片

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 159 kDa


      Exposure time: 30 seconds


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

      Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.

      Antibodies: ab2179 used for IP at 6 µg per reaction.

      Two lots tested (Lanes 1 and 2).

      Lane 3 is Control IgG.

      For blotting immunoprecipitated BLM, ab2179 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml

      Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 159 kDa


      Exposure time: 75 seconds


      Lysates prepared using NETN lysis buffer.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (38)

    发表研究结果有使用 ab2179?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2179 被引用在 38 文献中.

    • Lippert TP  et al. Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres. Nat Commun 12:512 (2021). PubMed: 33479235
    • Jiang W  et al. Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder. Biochim Biophys Acta Mol Basis Dis 1867:166106 (2021). PubMed: 33631320
    • Ercilla A  et al. Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication. Cell Rep 30:2416-2429.e7 (2020). PubMed: 32075739
    • Wood M  et al. TDP-43 dysfunction results in R-loop accumulation and DNA replication defects. J Cell Sci 133:N/A (2020). PubMed: 32989039
    • Mason-Osann E  et al. RAD54 promotes alternative lengthening of telomeres by mediating branch migration. EMBO Rep 21:e49495 (2020). PubMed: 32337843
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 17 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hek293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - protein G dynabeads, 10 microliters
    Specification
    hek293
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Edyta Marcon

    Verified customer

    提交于 Apr 30 2015

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TRex CHO ))
    Specification
    Chinese Hamster Ovary cells (TRex CHO )
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    提交于 Feb 05 2014

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (NTERA-2)
    Loading amount
    25 µg
    Specification
    NTERA-2
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 05 2013

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ntera2)
    Loading amount
    60000 cells
    Specification
    ntera2
    Treatment
    siRNA of Bloom
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Faisal Alzahrani

    Verified customer

    提交于 Oct 18 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon carcinoma cells)
    Specification
    Colon carcinoma cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    提交于 Oct 08 2012

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Sample
    Human Cell lysate - whole cell (Colon carcinoma)
    Specification
    Colon carcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    提交于 Oct 08 2012

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse testis)
    Loading amount
    100 µg
    Specification
    mouse testis
    Treatment
    Irradiation - 20 gy
    Gel Running Conditions
    Reduced Denaturing (8% Precast gel (Invitrogen))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 12 2012

    Immunocytochemistry abreview for Anti-Blooms Syndrome Protein Blm antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (mouse testis)
    Specification
    mouse testis
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 12 2012

    Immunocytochemistry abreview for Anti-Blooms Syndrome Protein Blm antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cell (HEK293-T)
    Specification
    HEK293-T
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 05 2011

    Question

    DESCRIPTION OF THE PROBLEM Non-specific staining
    SAMPLE Immortalized Human Bloom Syndrome cell line (6040 hTERT)
    PRIMARY ANTIBODY ab2179 dilutions, 1:100, 1:200, and 1:400 incubated 1hr, wash: 3X 5 min in 0.2% tween-20 in pbs
    DETECTION METHOD Fluorescent microscope
    POSITIVE AND NEGATIVE CONTROLS USED Positive Control: BJ hTERT (WT) Negative Control: 6040 hTERT (BS line)
    ANTIBODY STORAGE CONDITIONS 4C
    FIXATION OF SAMPLE 4% Paraformaldehyde
    PERMEABILIZATION STEP 1min in .2% Tween-20 in PBS
    BLOCKING CONDITIONS Blocking Buffer: 1% BSA, 0.2% tween-20, 0.02% azide in PBS 30 min at 37C
    SECONDARY ANTIBODY Invitrogen: Alexa Fluor 488 goat anti-rabbit IgG 1:400 dilution, 1hr at 37C wash: 3X 5 min in 0.2% tween-20 in pbs
    HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1
    HAVE YOU RUN A NO PRIMARY CONTROL? Yes
    DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
    ADDITIONAL NOTES I've attached a picture of the IF I obtained on my BS cell line (negative control) Blue: dapi, Green: Blm ab2179. I've looked through the literature and haven't been able to find anyone using any of the BLM antibodies against a BS cell line. Has this or any of the BLM antibodies been tested on Bloom Syndrome cell lines?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2012

    Answer

    Thank you for contacting Abcam regarding ab2179.

    I am sorry that you have been experiencing difficulties with this antibody in ICC. I have reviewed the protocol information you provided and would like to make some suggestions to improve your results with this antibody.

    Regarding your question about your cells, at this time we have not tested nor received feedback from anyone who has used this antibody in a Bloom Syndrome cell line. As a positive control, I would recommend testing HeLa cells treated with 15uM Bleomycin for 2 hours as this produces a positive result with the antibody.

    In terms of protocol modifications, I would recommend the following:

    Since this is a nuclear protein, I would recommend triton X-100 to permeabilize - try 0.1% in PBS for 10 min. If you continue to use Tween-20 I would increase this to at least 10 min. Alternatively I would recommend fixation with acetone, which will both fix and permeabilize the cells.

    The blocking step seems sufficient, but I would increase the incubation with the primary antibody to at least 2 hours, although ideally overnight at 4oC.

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions or if these suggestions do not improve your results with this antibody. The antibody is guaranteed under our Abpromise for WB and I would be happy to offer a replacement or credit if we determine the antibody is not working properly.

    Read More

    Abcam Scientific Support

    回复于 Mar 13 2012

    1-10 of 17 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有