Anti-Blooms Syndrome Protein Blm 抗体

• IP

Supplier Data

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (AB2179)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.

Antibodies : ab2179 used for IP at 6 μg per reaction.

Two lots tested (Lanes 1 and 2).

Lane 3 is Control IgG.

For blotting immunoprecipitated BLM, ab2179 was used at 0.1 μg/ml. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

Predicted band size: 159 kDa

false

• WB

Supplier Data

Western blot - Anti-Blooms Syndrome Protein Blm antibody (AB2179)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/mL

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 15 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 5:

A549 (Human lung carcinoma cell line) whole cell lysate at 15 µg

Predicted band size: 159 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-Blooms Syndrome Protein Blm antibody (AB2179)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/mL

Lane 1:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 159 kDa

false

Exposure time: 75s