AB3359

Anti-Blood Group Lewis y 抗体 [F3]

Name: Blood Group Lewis y抗体[F3] (ab3359)| Abcam中文官网
Brand: Abcam Limited
SKU: AB3359
Price: 4769 CNY
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Anti-Blood Group Lewis y antibody [F3]

(1 Review)

(24 Publications)

Mouse Monoclonal Blood Group Lewis y antibody. Suitable for IHC-P and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Cell preparation containing Blood Group Lewis y protein.

查看别名

Lewis Y blood group

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tumor tissue labeling Blood Group Lewis y with ab3359 at 1/40 dilution for 20 minutes at room temperature.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tumor tissue labeling Blood Group Lewis y with ab3359 at 1/40 dilution for 20 minutes at room temperature.

IHC-P

AbReview72077****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde-fixed human colon tissue permeabilized with 0.05% Tween 20. Stained with ab3359 at 1/50 dilution. Secondary antibody used was Alexa fluor® 647 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. Blocking was done with Sea Block for 30 minutes at 22°C. The sample was incubated with the primary antibody and Sea Block for 14 hours at 4°C. Antigen retrieval method was heat mediated, ab64236 100x Citrate Buffer pH 6.0.

This image is courtesy of an Abreview submitted by David Ivancic

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

亚型

IgM

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/40", "IHCP-species-notes": "<p></p>" } } }

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产品详情

Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

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性能和储存信息

形式

Liquid

纯化工艺

Precipitation Ammonium Sulphate

存储溶液

Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Blood Group Lewis y also known as Le^y or the Lewis y antigen is a carbohydrate antigen found on the surface of red blood cells and certain epithelial tissues. It is a fucosylated oligosaccharide structure specifically an f3-Lewis structure with a molecular mass around 725 Daltons. The Lewis y antigen is expressed most strongly in the epithelial cells of the gastrointestinal tract lungs and urinary tract. It also appears in the endothelial cells of blood vessels and some tumor cells where it can act as a tumor-associated carbohydrate antigen.

Biological function summary

The Lewis y antigen plays a significant role in cell adhesion and cell-cell recognition. It becomes engaged in the regulation of cellular interaction being part of complex glycoproteins and glycolipids on cell membranes. These structures serve as ligands for selectins which are adhesion molecules involved in immune responses. The presence of Lewis y antigen can influence cellular behavior and interactions impacting immune response and tumor progression.

Pathways

The Lewis y antigen is involved in the selectin-mediated signaling pathway and glycosylation processes. These pathways relate to its role in cell adhesion and immune system navigation where it interacts with selectin proteins such as L-selectin and E-selectin. Glycosyltransferases enzymes part of the glycosylation process modify the Lewis y antigen forming structures essential for proper cellular recognition and communication in various physiological contexts.

The Lewis y antigen has associations with certain types of cancer such as epithelial ovarian and gastric cancers. It often becomes overexpressed in tumor cells making it a marker for cancer progression and a target for cancer immunotherapy. The upregulation of the Lewis y antigen might interact with molecules like HER2/neu a protein commonly overexpressed in cancer cells contributing to aggressive tumor behavior and metastasis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Blood Group Lewis y

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文献 (24)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35052824

2022

The Effects of and Knockout Mutations on the Structure and Function of Lipopolysaccharide in Strain 26695.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Ning Liu,Kai-Wen Teng,Yongyu Chew,Po-Chuan Wang,Tram Thi Hong Nguyen,Mou-Chieh Kao

Methods in molecular biology (Clifton, N.J.) 2460:33-44 PubMed34972929

2022

Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Purohit,Jin-Xiong She

Journal of orthopaedic translation 21:129-135 PubMed32309138

2020

Association between the ABO blood group and primary knee osteoarthritis: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Changchuan Li,Nengtai Ouyang,Xiuju Wang,Anjing Liang,Yingqian Mo,Shixun Li,Junxiong Qiu,Guibin Fang,Yuan Fu,Bin Song,Zhong Chen,Yue Ding

BMC research notes 12:607 PubMed31547886

2019

Characterization of human norovirus binding to gut-associated bacterial ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Erin A Almand,Matthew D Moore,Lee-Ann Jaykus

International journal of molecular medicine 43:1687-1698 PubMed30816446

2019

Co‑expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Liu,Qi Liu,Yanyan Wang,Miao Liu,Yue Qi,Jian Gao,Bei Lin

Glycobiology 29:137-150 PubMed30476078

2018

Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Athanasios Blanas,Lenneke A M Cornelissen,Maximilianos Kotsias,Joost C van der Horst,Henri J van de Vrugt,Hakan Kalay,Daniel I R Spencer,Rad P Kozak,Sandra J van Vliet

International journal of oncology 53:1774-1786 PubMed30066907

2018

Lewis(y) antigen-mediated positive feedback loop induces and promotes chemotherapeutic resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Liu,Mingjun Zheng,Yue Qi,Huimin Wang,Miao Liu,Qing Liu,Bei Lin

Nature communications 9:258 PubMed29343722

2018

Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Purohit,Tiehai Li,Wanyi Guan,Xuezheng Song,Jing Song,Yanna Tian,Lei Li,Ashok Sharma,Boying Dun,David Mysona,Sharad Ghamande,Bunja Rungruang,Richard D Cummings,Peng George Wang,Jin-Xiong She

Cell death and differentiation 24:2161-2172 PubMed28914881

2017

MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and α1,3-fucosylation.

Applications

Species

Unspecified reactive species

Qin Zheng,Dandan Zhang,Y U Yang,Xinyuan Cui,Jiaqi Sun,Caixia Liang,Huamin Qin,Xuesong Yang,Shuai Liu,Qiu Yan

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:1010428317711655 PubMed28671040

2017

High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liancheng Zhu,Huilin Feng,Shan Jin,Mingzi Tan,Song Gao,Huiyu Zhuang,Zhenhua Hu,Huimin Wang,Zuofei Song,Bei Lin

View all publications

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Anti-Blood Group Lewis y 抗体 [F3]

Anti-Blood Group Lewis y antibody [F3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性