Anti-Blood Group Lewis b抗体[2-25LE] (ab3968)
Key features and details
- Mouse monoclonal [2-25LE] to Blood Group Lewis b
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Blood Group Lewis b抗体[2-25LE] -
描述
小鼠单克隆抗体[2-25LE] to Blood Group Lewis b -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, Flow Cyt, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Blood Group Lewis b. Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
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阳性对照
- IHC: Human colon adenocarcinoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
2-25LE -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 23144050
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 17202152
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IHC-P |
Use a concentration of 5 - 10 µg/ml.
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说明 |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 23144050 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. PubMed: 17202152 |
IHC-P
Use a concentration of 5 - 10 µg/ml. |
靶标
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相关性
Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. -
细胞定位
Plasma membrane - adsorbed onto the surface of erythrocytes. -
数据库链接
- Entrez Gene: 4586 Human
- SwissProt: P98088 Human
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别名
- gastric mucin antibody
- leB antibody
- lewis antigen system antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab3968 被引用在 18 文献中.
- Purohit S & She JX Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies. Methods Mol Biol 2460:33-44 (2022). PubMed: 34972929
- Chikadze N et al. Prevalence of high affinity naturally occurring IgG2 antibodies against human chorionic gonadotropin and its subunits in patients with ovarian cyst. Immunobiology 227:152273 (2022). PubMed: 36088866
- Almand EA et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Cuello HA et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930