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Cardiovascular Blood Blood Cell Antigens

Anti-Blood Group H ab antigen抗体[87-N] (ab24222)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [87-N] to Blood Group H ab antigen
  • Suitable for: ELISA, IP, Agglutination
  • Reacts with: Human
  • Isotype: IgM

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概述

  • 产品名称

    Anti-Blood Group H ab antigen抗体[87-N]
    参阅全部 Blood Group H ab antigen 一抗
  • 描述

    小鼠单克隆抗体[87-N] to Blood Group H ab antigen
  • 宿主

    Mouse
  • 经测试应用

    适用于: ELISA, IP, Agglutinationmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    H-antigen on red blood cells (Human).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    87-N
  • 同种型

    IgM
  • 研究领域

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgM H&L (ab9167)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab24222于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Agglutination
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Agglutination
Use at an assay dependent concentration.

实验方案

  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab24222?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab24222 尚未被引用在任何文献中。

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blood Group H ab antigen antibody [87-N]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana Cell Conditioning 1 buffer
Specification
liver
Blocking step
OptiView HQ Universal Linker as blocking agent for 8 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 13 2022

Question

Will the Anti-Blood Group H ab antigen antibody [87-N] (ab24222) only cross-react with H1 and H2 antigens and not A/B antigens?

Read More

Abcam community

Verified customer

Asked on Jun 20 2013

Answer

Unfortunately, the only information I managed to obtain is that the anti-H has a titer of 1/256 with 0 RBC, 1/4 with A and B RBC and +/- with AB group in direct tube test.
Anti-H binds H in saliva.

Read More

Guillaume Boucher

Abcam Scientific Support

回复于 Jun 20 2013

Question

How was the specificity of these antibodies tested (in IHC-P or WB)
ab3354: IHC-P
ab24222: WB
ab2521: IHC-P

Read More

Abcam community

Verified customer

Asked on May 22 2012

Answer

Thank you for your patience.

Regarding ab24222, the supplying lab sent me the following information:

OnlyELISA, and IP have been tested. This antibody is used in direct agglutination tests to determine antigen presence in biological fluids. The specificity was determined by lack of agglutination of antibody when pretreated by soluble antigen.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 May 22 2012

Question

How was the specificity of these antibodies tested (in IHC-P or WB)
ab3354: IHC-P
ab24222: WB
ab2521: IHC-P

Read More

Abcam community

Verified customer

Asked on May 18 2012

Answer

Thank you for contacting us.

I have received the following information:

ab2521: the IHC(P) application was tested on human colon carcinoma tissue. The paraffin-embedded sections were deparaffinized by xylene, hydrated by 100%, 90%, 75% ethanol and distilled water, incubated with 5% H2O2, blocked with porcine serum devoid of ABO-reactive antibodies and then incubated with primary antibody, secondary antibody, and the signal was developed by DAB.

ab3354: This products has been tested on FFPE normal human kidney tissue. We recommend using a 20min incubation of the primary @ 1:40 dilution and no antigen retrieval should be needed.
I am inquiring about more specificity-testing information with the lab.

ab24222: Unfortunately, the antibodywas not tested for WB, just in ELISA type format. Here, I am also inquiring about more specificity-testing information with the supplying lab for ELISA. I have updated our datasheet and removed WB. I am sorry for the incorrect information.

I will be in touch with more details, buthope this information is already of some help to you.

Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 May 19 2012

Question

Dear Sir or Ma’am, Thank you so much for all of the help I received from your technical support team when I called today to inquire about an antibody purchase I’d like to make.  I wanted to ask a couple more questions before placing my order:  For the product Ab 24222, it has not been tested yet for IHC-P in humans, which is the purpose for which I’d like to use it.  It was mentioned that if I did an abreview, we could get a coupon for a free antibody in the future.  What are the steps to enter into that agreement?  Also for products Ab 2521 and Ab2524, there are not pictures or western blots to show the antibody quality.  We plan to use these too for IHC-P in humans, for which they’ve been guaranteed.  If I order them and they don’t work well, what is entailed in that guarantee and what process is in place?  I very much appreciate your time in answering these questions.  Sincerely,  

Read More

Abcam community

Verified customer

Asked on Dec 05 2011

Answer

Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 and ab2524 both have been tested for IHC-P (however no image available yet) so testing discount can also be requested. We can offer you one discount code for one product only first; however after publishing the Abreview we can certainly offer a new discount code for the other product you wish to test.   You can find some further information about our testing scheme at this site: The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me. Please do let me know if you wish to test any of these products.

Read More

Abcam Scientific Support

回复于 Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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