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Anti-Blood Group A Antigen抗体[HE-193] (ab2521)

Submit a review Q&A (3)References (4)

Key features and details

  • Mouse monoclonal [HE-193] to Blood Group A Antigen
  • Suitable for: IHC-P, Agglutination
  • Reacts with: Human
  • Isotype: IgM

概述

  • 产品名称

    Anti-Blood Group A Antigen抗体[HE-193]
    参阅全部 Blood Group A Antigen 一抗

  • 描述

    小鼠单克隆抗体[HE-193] to Blood Group A Antigen

  • 宿主

    Mouse

  • 特异性

    This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssman antigen.

  • 经测试应用

    适用于: IHC-P, Agglutinationmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Constituent: Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 纯化说明

    Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.

  • 克隆

    单克隆

  • 克隆编号

    HE-193

  • 骨髓瘤

    unknown

  • 同种型

    IgM

  • 轻链类型

    unknown

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2521于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
Agglutination
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
Agglutination
Use at an assay dependent concentration.

靶标

  • 相关性

    Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

  • 细胞定位

    Cell Membrane

  • 数据库链接

    文献 (4)

    发表研究结果有使用 ab2521？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2521 被引用在 4 文献中.

    • Säljö K  et al. Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands. Int J Mol Sci 22:N/A (2021). PubMed: 34208903
    • Li C  et al. Association between the ABO blood group and primary knee osteoarthritis: A case-control study. J Orthop Translat 21:129-135 (2020). PubMed: 32309138
    • Johansson MM  et al. Characterization of moose intestinal glycosphingolipids. Glycoconj J 32:393-412 (2015). PubMed: 26104834
    • Nemec M  et al. Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang 52:125-8 (1987). PubMed: 2440181

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    How was the specificity of these antibodies tested (in IHC-P or WB)
    ab3354: IHC-P
    ab24222: WB
    ab2521: IHC-P

    Read More
    Answer

    Thank you for contacting us.

    I have received the following information:

    ab2521: the IHC(P) application was tested on human colon carcinoma tissue. The paraffin-embedded sections were deparaffinized by xylene, hydrated by 100%, 90%, 75% ethanol and distilled water, incubated with 5% H2O2, blocked with porcine serum devoid of ABO-reactive antibodies and then incubated with primary antibody, secondary antibody, and the signal was developed by DAB.

    ab3354: This products has been tested on FFPE normal human kidney tissue. We recommend using a 20min incubation of the primary @ 1:40 dilution and no antigen retrieval should be needed.
    I am inquiring about more specificity-testing information with the lab.

    ab24222: Unfortunately, the antibodywas not tested for WB, just in ELISA type format. Here, I am also inquiring about more specificity-testing information with the supplying lab for ELISA. I have updated our datasheet and removed WB. I am sorry for the incorrect information.

    I will be in touch with more details, buthope this information is already of some help to you.

    Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear Sir or Ma’am, Thank you so much for all of the help I received from your technical support team when I called today to inquire about an antibody purchase I’d like to make.  I wanted to ask a couple more questions before placing my order:  For the product Ab 24222, it has not been tested yet for IHC-P in humans, which is the purpose for which I’d like to use it.  It was mentioned that if I did an abreview, we could get a coupon for a free antibody in the future.  What are the steps to enter into that agreement?  Also for products Ab 2521 and Ab2524, there are not pictures or western blots to show the antibody quality.  We plan to use these too for IHC-P in humans, for which they’ve been guaranteed.  If I order them and they don’t work well, what is entailed in that guarantee and what process is in place?  I very much appreciate your time in answering these questions.  Sincerely,  

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. We can offer testing discount for untested species/application and also for images for tested application.   I can confirm that ab24222 has not been tested yet in IHC; whilst ab2521 and ab2524 both have been tested for IHC-P (however no image available yet) so testing discount can also be requested. We can offer you one discount code for one product only first; however after publishing the Abreview we can certainly offer a new discount code for the other product you wish to test.   You can find some further information about our testing scheme at this site: The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Follow these easy steps for a 100% testing discount: Email technical@abcam.com or us.technical@abcam.com and tell our Scientific Support team the product, application, and species you are interested in testing Our scientists will email you a personal discount code to give you a 100% discount on your next product Purchase the product at regular price Test the product in your species and application Submit your results: www.abcam.com/abreviews Once the Abreview is submitted, the discount code will become active Place a new order by phone, fax, or web and include your 100% testing discount code If you need any further assistance in the future, please do not hesitate to contact me. Please do let me know if you wish to test any of these products.

    Read More

    Question

    When you say that it is a tissue culure supernatant you mean that no buffer is added to it so as to extract the antibodies? I have read in a manual that tissue culture supernatants are stored in 1/20 volume 1M tris buffer solution, pH=8. do you keep your antibodies under these conditions? thanks.

    Read More
    Answer

    Thank you for your enquiry. The format of ab2521 is condensed supernatant with 15 mM sodium azide. No more supplements or buffers are added or necessary. We do not store this antibody in 1/20 volume 1M tris buffer solution, pH 8. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

    Read More

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