重组Anti-BLNK抗体[Y491] (ab32418)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y491] to BLNK
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-BLNK抗体[Y491]
参阅全部 BLNK 一抗 -
描述
兔单克隆抗体[Y491] to BLNK -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human BLNK aa 400-500 (C terminal). The exact sequence is proprietary.
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阳性对照
- Raji cell lysate and human spleen tissue slides.
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常规说明
The production method for this product has been changed from hybridoma to recombinant on 18th June 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y491 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32418于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/20.
|
|
WB |
1/1000.
|
说明 |
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IP
1/20. |
WB
1/1000. |
靶标
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功能
Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis. -
组织特异性
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. -
疾病相关
Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
序列相似性
Contains 1 SH2 domain. -
翻译后修饰
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. -
细胞定位
Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction. - Information by UniProt
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数据库链接
- Entrez Gene: 29760 Human
- Entrez Gene: 17060 Mouse
- Entrez Gene: 499356 Rat
- Omim: 604515 Human
- SwissProt: Q8WV28 Human
- SwissProt: Q9QUN3 Mouse
- SwissProt: Q4KM52 Rat
- Unigene: 665244 Human
see all -
别名
- AGM4 antibody
- B cell adapter containing SH2 domain protein antibody
- B cell adapter containing Src homology 2 domain protein antibody
see all
图片
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BLNK was immunoprecipitated from 10µg of Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab32418 at 1/20 dilution (0.5 μg in 0.35 mg lysates). Western blot was performed from the immunoprecipitate using ab32418 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg
Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32418 in Raji whole cell lysate
Exposure time: 10 seconds
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All lanes : Anti-BLNK antibody [Y491] (ab32418) at 1/1000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 7 seconds; Lane 2, 40 seconds; Lane 3, 60 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab32418 被引用在 1 文献中.
- Wei SY et al. Curcumin alleviates experimental colitis via a potential mechanism involving memory B cells and Bcl-6-Syk-BLNK signaling. World J Gastroenterol 28:5865-5880 (2022). PubMed: 36353208