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AB2230

Anti-BLNK抗体

Anti-BLNK antibody

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Goat Polyclonal BLNK antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BLNK aa 400 to C-terminus.

查看别名

BASH, SLP65, BLNK, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65

2 Images
Western blot - Anti-BLNK antibody (AB2230)
  • WB

Unknown

Western blot - Anti-BLNK antibody (AB2230)

ab2230 staining (4μg/ml) of Daudi lysate (RIPA buffer, 30μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour.

All lanes:

Western blot - Anti-BLNK antibody (ab2230) at 4 µg/mL

All lanes:

Cell lysate prepared from human Daudi cells at 35 µg

Predicted band size: 50 kDa

Observed band size: 70 kDa

true

Western blot - Anti-BLNK antibody (AB2230)
  • WB

Unknown

Western blot - Anti-BLNK antibody (AB2230)

All lanes:

Western blot - Anti-BLNK antibody (ab2230) at 1 µg/mL

Lane 1:

Cell lysate prepared from HEK293 mock transfected cells

Lane 2:

Cell lysate prepared from HEK293 overexpressing BLNK

Predicted band size: 50 kDa

Observed band size: 50 kDa

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Synthetic Peptide within Human BLNK aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WV28

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.05% Tris
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
See full target information BLNK
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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