生物素Anti-Wnt3a抗体(ab54479)
Key features and details
- Biotin Rabbit polyclonal to Wnt3a
- Suitable for: Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Wnt3a抗体
参阅全部 Wnt3a 一抗 -
描述
生物素兔多克隆抗体to Wnt3a -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant full length protein (Human),>98% pure
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯化说明
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Use 100 µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a. |
| 说明 |
|---|
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. Use 100 µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a. |
靶标
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功能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
组织特异性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
序列相似性
Belongs to the Wnt family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
图片
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Sandwich ELISA - Biotin Anti-Wnt3a antibody (ab54479)
ab54479 detecting Wnt3a by sanwich ELISA using 100 μl/well antibody solution. Samples were incubated with primary antibody ab54479 (1.0 μg/ml) and capture antibody Polyclonal Anti-Human WNT-3a. Allows detection of at least 0.2 – 0.4 ng/well of recombinant hWNT-3a.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab54479 被引用在 2 文献中.
- Ling L et al. Synergism between Wnt3a and heparin enhances osteogenesis via a phosphoinositide 3-kinase/Akt/RUNX2 pathway. J Biol Chem 285:26233-44 (2010). PubMed: 20547765
- Kwon HS et al. Myocilin is a modulator of Wnt signaling. Mol Cell Biol 29:2139-54 (2009). ELISA ; Mouse . PubMed: 19188438
