Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

生物素Anti-VCAM1抗体[M/K-2] (ab24942)

Submit a review Q&A (1)
Flow Cytometry - Biotin Anti-VCAM1 antibody [M/K-2] (ab24942)

    Key features and details

    • Biotin Rat monoclonal [M/K-2] to VCAM1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: Biotin
    • Isotype: IgG1

    概述

    • 产品名称

      生物素Anti-VCAM1抗体[M/K-2]
      参阅全部 VCAM1 一抗

    • 描述

      生物素大鼠单克隆抗体[M/K-2] to VCAM1

    • 宿主

      Rat

    • 偶联物

      Biotin

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to VCAM1. BALB/3T3 and +/+2.4 cells

    • 阳性对照

      • Flow Cyt: BALB/c mouse bone marrow cells.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab24942于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 1µg for 106 cells.

    ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    说明
    Flow Cyt
    Use 1µg for 106 cells.

    ab18403 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

    • 组织特异性

      Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

    • 序列相似性

      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

    • 结构域

      Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.

    • 翻译后修饰

      Sialoglycoprotein.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD106 antibody
        • CD106 Antigen antibody
        • INCAM 100 antibody
        • INCAM-100 antibody
        • L1CAM antibody
        • MGC99561 antibody
        • V-CAM 1 antibody
        • Vascular Cell Adhesion Molecule 1 antibody
        • Vascular cell adhesion protein 1 antibody
        • VCAM 1 antibody
        • VCAM-1 antibody
        • VCAM1 antibody
        • VCAM1_HUMAN antibody
        see all

      图片

      • Flow Cytometry - Biotin Anti-VCAM1 antibody [M/K-2] (ab24942)
        Flow Cytometry - Biotin Anti-VCAM1 antibody [M/K-2] (ab24942)

        BALB/c mouse bone marrow cells were stained with Anti-VCAM1 antibody [M/K-2] (Biotin) (ab24942) and Rat Anti-Mouse Ly-6G/Ly-6C-PE followed by Streptavidin-FITC.

      文献 (0)

      发表研究结果有使用 ab24942？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab24942 尚未被引用在任何文献中。

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      Inquiry: Hi, I have tested this antibody a couple of times using an ICC protocol for mouse aortic endothelial cells that were stimulated with TNF alpha to express VCAM1. The staining I am getting is very weak and I just wondered if you have any suggestions for a protocol to follow and the best concentration of antibody to use. My protocol was as follows Remove culture medium from each well and wash twice with PBS Add 1ml 4% paraformaldehyde solution in PBS for 15-20mins at room temperature Wash cells twice with PBS and then cover with 1ml wash buffer ( 0.1% BSA in PBS)(Coverslips can be stored in fridge for up to 3 months or stained immediately) Wash the coverslips containing the fixed cells two times with 1ml wash buffer. Blocking step – incubate cells for 45mins with either 1% BSA in PBS or 10% serum from which secondary antibody is raised. 1 Incubate cells with primary antibody tried 1:100, 1:200 and 1:500 very weak staining at 1:100 and 1:200 but not much at 1:500 (diluted in 1% BSA) in humidified chamber overnight at 4⁰C Decant the solution and wash the cells 3 times with PBS 0.1% BSA Incubate cells with fluorescent secondary antibody Streptavidin alexa fluor 488 1:200 for 1 hour at room temperature in the dark. Decant secondary antibody solution and wash with PBS 3 x 5 mins in the dark. Incubate cells with DAPI for 1 min Rinse with PBS 4 x 5 min washes Remove the coverslips from wells and blot to remove any water, dispense 1 drop of anti-fade mounting medium onto microscope slide and then mount coverslip with cells facing towards the microscope slide. Either store at -20C or visualise under fluorescent microscope. I also wondered if this antibody has been tested in paraffin embedded or frozen sections and what conditions you would recommend for antigen retrieval and what concentration of primary antibody to use. Any advice you can give would be greatly appreciated Best Wishes

      Read More
      Answer

      Thank you for your enquiry regarding ab24942 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
      I can confirm that ab24942 has been tested and characterized for ICC/IF but not for Immunohistochemistry (IHC-Fr or IHC-P). Though, it may well work in IHC but we have no data to share.
      The protocol looks fine to me but I would make a few comments/suggestions:
      1) Fresh samples:
      It may be worth using freshly prepared and fixed samples. Try different fixatives such as ice-cold methanol or ethanol (5 mins).
      2) Detection system:
      Does the detection system work fine? Have you used it successfully with another primary antibody?
      3) Positive control:
      Brain, kidney, placenta, tonsil cells can be used as positive control. You may wish to consult this site to get some further information about the expression profile of VCAM1 in different cells/tissues:
      http://www.proteinatlas.org/ENSG00000162692
      I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com